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Int. J. Mol. Sci. 2017, 18(6), 1199; doi:10.3390/ijms18061199

Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen

1
Deptartment of Molecular Biology, University of Salzburg, A-5020 Salzburg, Austria
2
Faculty of Agricultural Sciences, Georg-August-Universität Göttingen, D-37075 Göttingen, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Joseph V. Moxon
Received: 28 April 2017 / Revised: 24 May 2017 / Accepted: 1 June 2017 / Published: 5 June 2017
(This article belongs to the Special Issue Proteolysis in Allergic Sensitization and Th2 Response)
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Abstract

Pollen is one of the most common causes of allergy worldwide, making the study of their molecular composition crucial for the advancement of allergy research. Despite substantial efforts in this field, it is not yet clear why some plant pollens strongly provoke allergies while others do not. However, proteases and protease inhibitors from allergen sources are known to play an important role in the development of pollen allergies. In this study, we aim to uncover differences in the transcriptional pattern of proteases and protease inhibitors in Betula verrucosa and Pinus sylvestris pollen as models for high and low allergenic potential, respectively. We applied RNA sequencing to Betula verrucosa and Pinus sylvestris pollen. After de-novo assembly we derived general functional profiles of the protein coding transcripts. By utilization of domain based functional annotation we identified potential proteases and protease inhibitors and compared their expression in the two types of pollen. Functional profiles are highly similar between Betula verrucosa and Pinus sylvestris pollen. Both pollen contain proteases and inhibitors from 53 and 7 Pfam families, respectively. Some of the members comprised within those families are implicated in facilitating allergen entry, while others are known allergens themselves. Our work revealed several candidate proteins which, with further investigation, represent exciting new leads in elucidating the process behind allergic sensitization. View Full-Text
Keywords: pollen transcriptome; protease; protease inhibitor; allergenicity pollen transcriptome; protease; protease inhibitor; allergenicity
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MDPI and ACS Style

Höllbacher, B.; Schmitt, A.O.; Hofer, H.; Ferreira, F.; Lackner, P. Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen. Int. J. Mol. Sci. 2017, 18, 1199.

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