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Int. J. Mol. Sci. 2017, 18(6), 1153; doi:10.3390/ijms18061153

The Methods of Choice for Extracellular Vesicles (EVs) Characterization

1
Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland
2
Tissue Biology Research Unit, Department of Surgery, University Children’s Hospital Zurich, 8032 Zurich, Switzerland
3
Department of Research of Biophysical Microstructure, Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Thomas Ritter, Matthew Griffin and Aideen Ryan
Received: 28 February 2017 / Revised: 23 May 2017 / Accepted: 24 May 2017 / Published: 29 May 2017
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Abstract

In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization. View Full-Text
Keywords: extracellular vesicles (EVs); exosomes; microvesicles (MVs); flow cytometry; dynamic light scattering (DLS); stimulated emission depletion microscopy (STED); nanoparticle tracking analysis (NTA); transmission electron microscopy (TEM); cryo-electron microscopy (Cryo-EM); atomic force microscopy (AFM) extracellular vesicles (EVs); exosomes; microvesicles (MVs); flow cytometry; dynamic light scattering (DLS); stimulated emission depletion microscopy (STED); nanoparticle tracking analysis (NTA); transmission electron microscopy (TEM); cryo-electron microscopy (Cryo-EM); atomic force microscopy (AFM)
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MDPI and ACS Style

Szatanek, R.; Baj-Krzyworzeka, M.; Zimoch, J.; Lekka, M.; Siedlar, M.; Baran, J. The Methods of Choice for Extracellular Vesicles (EVs) Characterization. Int. J. Mol. Sci. 2017, 18, 1153.

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