Emergence of CD26+ Cancer Stem Cells with Metastatic Properties in Colorectal Carcinogenesis
Abstract
:1. Introduction
2. Results
2.1. Primary Tumors with Confirmed or Suspected Metastases Harbor a Relatively High Proportion of CD26+ Cancer Stem Cell Subpopulation
2.2. PIK3CA Inhibitor Decreases the Maintenance of CD26+ Cell Population in Culture
2.3. TP53 Knockdown Increases the Maintenance of CD26+ Cell Population in Culture
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Flow Cytometry and Fluorescent-Activated Cell Sorting (FACS)
4.3. PIK3CA Inhibitor Assay
4.4. Knockdown of P53
4.5. Clinical Specimens
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Conflicts of Interest
References
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Patient | Gender and Age | CD26+ Cells % | Stage at Surgery | Tumour Size (cm) | Degree of Differentiation | Metastasis Diagnosed or Suspected | Survival Status | Metastasis-Free Survival (Months) | Overall Survival (Months) |
---|---|---|---|---|---|---|---|---|---|
1 | M/81 | 0.2 | III | 9 | Moderate | Liver | Deceased | 5.40 | 9.70 |
2 | F/80 | 0.3 | I | 5 | Moderate | None | Alive | 39.23 | 39.23 |
3 | F/68 | 0.4 | IIA | 10 | Moderate | None | Alive | 36.20 | 36.20 |
4 | M/71 | 0.5 | IIIC | 5 | Moderate | Peritoneum and bone | Deceased | 13.97 | 19.83 |
5 | M/79 | 0.6 | IIA | 11 | Poor | None | Alive | 33.17 | 33.17 |
6 | M/83 | 3.3 | IV | 12 | Moderate | Lung | Deceased | NA | 19.47 |
7 | M/74 | 8.2 | IV | 5 | Poor | Liver | Deceased | NA | 51.97 |
8 | F/56 | 9.6 | IV | 5.5 | Moderate | Liver | Deceased | NA | 29.67 |
9 | M/68 | 9.9 | IV | 9 | Moderate | Lung and liver | Alive | NA | 47.50 |
10 | F/84 | 12.7 | IIA | 10 | Moderate | Suspected lung metastasis | Alive | 21.30 | 31.23 |
11 | M/78 | 13.2 | IIA | 15 | Moderate | Liver | Deceased | 5.10 | 5.17 |
Number of Patients | Confirmed or Suspected Metastases | ||
---|---|---|---|
Absent | Present | ||
CD26+ cell proportion | low | 3 | 2 |
high | 0 | 6 |
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Cheung, A.H.-K.; Iyer, D.N.; Lam, C.S.-C.; Ng, L.; Wong, S.K.M.; Lee, H.-S.; Wan, T.; Man, J.; Chow, A.K.M.; Poon, R.T.; et al. Emergence of CD26+ Cancer Stem Cells with Metastatic Properties in Colorectal Carcinogenesis. Int. J. Mol. Sci. 2017, 18, 1106. https://doi.org/10.3390/ijms18061106
Cheung AH-K, Iyer DN, Lam CS-C, Ng L, Wong SKM, Lee H-S, Wan T, Man J, Chow AKM, Poon RT, et al. Emergence of CD26+ Cancer Stem Cells with Metastatic Properties in Colorectal Carcinogenesis. International Journal of Molecular Sciences. 2017; 18(6):1106. https://doi.org/10.3390/ijms18061106
Chicago/Turabian StyleCheung, Alvin Ho-Kwan, Deepak Narayanan Iyer, Colin Siu-Chi Lam, Lui Ng, Sunny K. M. Wong, Hung-Sing Lee, Timothy Wan, Johnny Man, Ariel K. M. Chow, Ronnie T. Poon, and et al. 2017. "Emergence of CD26+ Cancer Stem Cells with Metastatic Properties in Colorectal Carcinogenesis" International Journal of Molecular Sciences 18, no. 6: 1106. https://doi.org/10.3390/ijms18061106