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Int. J. Mol. Sci. 2017, 18(5), 958; doi:10.3390/ijms18050958

Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones

1
Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
College of Agriculture, Hainan University, Haikou 570228, China
3
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
4
Center for Metabolic Biology, Iowa State University, Ames, IA 50011, USA
5
Department of Biological Sciences, Mississippi State University, Starkville, MS 39762, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Setsuko Komatsu
Received: 23 January 2017 / Revised: 3 April 2017 / Accepted: 21 April 2017 / Published: 2 May 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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Abstract

Rubber elongation factor (REF) and small rubber particle protein (SRPP) are two key factors for natural rubber biosynthesis. To further understand the roles of these proteins in rubber formation, six different genes for latex abundant REF or SRPP proteins, including REF138,175,258 and SRPP117,204,243, were characterized from Hevea brasiliensis Reyan (RY) 7-33-97. Sequence analysis showed that REFs have a variable and long N-terminal, whereas SRPPs have a variable and long C-terminal beyond the REF domain, and REF258 has a β subunit of ATPase in its N-terminal. Through two-dimensional electrophoresis (2-DE), each REF/SRPP protein was separated into multiple protein spots on 2-DE gels, indicating they have multiple protein species. The abundance of REF/SRPP proteins was compared between ethylene and control treatments or among rubber tree clones with different levels of latex productivity by analyzing 2-DE gels. The total abundance of each REF/SRPP protein decreased or changed a little upon ethylene stimulation, whereas the abundance of multiple protein species of the same REF/SRPP changed diversely. Among the three rubber tree clones, the abundance of the protein species also differed significantly. Especially, two protein species of REF175 or REF258 were ethylene-responsive only in the high latex productivity clone RY 8-79 instead of in RY 7-33-97 and PR 107. Some individual protein species were positively related to ethylene stimulation and latex productivity. These results suggested that the specific protein species could be more important than others for rubber production and post-translational modifications might play important roles in rubber biosynthesis. View Full-Text
Keywords: comparative proteomics; Hevea brasiliensis; ethylene stimulation; latex productivity; rubber elongation factor; small rubber particle protein comparative proteomics; Hevea brasiliensis; ethylene stimulation; latex productivity; rubber elongation factor; small rubber particle protein
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MDPI and ACS Style

Tong, Z.; Wang, D.; Sun, Y.; Yang, Q.; Meng, X.; Wang, L.; Feng, W.; Li, L.; Wurtele, E.S.; Wang, X. Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones. Int. J. Mol. Sci. 2017, 18, 958.

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