Next Article in Journal
Radiation and Thyroid Cancer
Previous Article in Journal
Prevention of Colorectal Cancer by Targeting Obesity-Related Disorders and Inflammation
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(5), 910; doi:10.3390/ijms18050910

miR-103 Promotes Proliferation and Metastasis by Targeting KLF4 in Gastric Cancer

1
Department of Diagnostic Pathology, Weifang Medical University, Weifang 261053, China
2
Department of Pathology, Affiliated Hospital of Weifang Medical University, Weifang 261053, China
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 7 April 2017 / Revised: 19 April 2017 / Accepted: 23 April 2017 / Published: 26 April 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2888 KB, uploaded 26 April 2017]   |  

Abstract

MicroRNAs (miRNAs) play important roles in the cancer development and progression; overexpression of miR-103 has been identified in various tumors. However, its biological function and regulatory mechanism involved in modulation of human gastric cancer (GC) remain largely unknown. This study aimed to confirm clinical significance of miR-103 and investigate its biological role and underlying mechanism in GC. Real-time quantitative PCR (qRT-PCR) revealed miR-103 was highly expressed in GC tissues and cell lines. miR-103 expression was correlated closely with tumor size, Lauren’s classification, and lymph node metastasis. Importantly, Kaplan-Meier analysis revealed that high expression of miR-103 was significantly associated with poor overall survival and disease-free survival of GC patients. Downregulation of miR-103 by transfecting with miR-103 inhibitor significantly suppressed cell proliferation, induced apoptosis, inhibited migration and invasion in vitro and in vivo. Furthermore, miRNA target databases and luciferase reporter assay confirmed that Krüppel-like Factor-4 (KLF4) was a direct target of miR-103 in GC, and there was a significant inverse correlation between miR-103 and KLF4 expression in GC tissues. Moreover, KLF4 downregulation could rescue miR-103’s oncogenic effect on GC cell proliferation, apoptosis, migration, and invasion. Therefore, these results suggested that miR-103 overexpression could contribute to tumor progression by suppressing KLF4, and it might serve as a promising candidate for the prognosis of GC patients. View Full-Text
Keywords: gastric cancer; microRNA-103; KLF4; proliferation; metastasis gastric cancer; microRNA-103; KLF4; proliferation; metastasis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zheng, J.; Liu, Y.; Qiao, Y.; Zhang, L.; Lu, S. miR-103 Promotes Proliferation and Metastasis by Targeting KLF4 in Gastric Cancer. Int. J. Mol. Sci. 2017, 18, 910.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top