Next Article in Journal
Supervised Clustering of Adipokines and Hormonal Receptors Predict Prognosis in a Population of Obese Women with Type 1 Endometrial Cancer
Next Article in Special Issue
Comparative Study of Lectin Domains in Model Species: New Insights into Evolutionary Dynamics
Previous Article in Journal
First Insights into Human Fingertip Regeneration by Echo-Doppler Imaging and Wound Microenvironment Assessment
Previous Article in Special Issue
Anti-Neuroblastoma Properties of a Recombinant Sunflower Lectin
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(5), 1052; doi:10.3390/ijms18051052

Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells

1
Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan
2
National Institute of Advanced Industrial Science and Technology, 1-1-1 Umezono, Ibaraki 305-8568, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Els Van Damme
Received: 29 March 2017 / Revised: 5 May 2017 / Accepted: 10 May 2017 / Published: 13 May 2017
(This article belongs to the Special Issue Plant Lectins: From Model Species to Crop Plants)
View Full-Text   |   Download PDF [2990 KB, uploaded 13 May 2017]   |  

Abstract

Rice bran lectins, named as RBA1 and RBA2, were isolated from Oryza sativa in two chromatography steps: affinity chromatography and cation-exchange chromatography. RBA1 was found to be composed of a covalently linked heterodimer of 20- and 12-kDa subunits, and RBA2 was a noncovalently linked dimer of 12-kDa subunits. Both RBA1 and RBA2 bound to desialylated complex glycoproteins such as fetuin, α1-acid glycoprotein, and transferrin, and agalactosylated complex glycoproteins such as agalacto fetuin, agalacto-α1-acid glycoprotein, and agalacto-transferrin, in addition to chitooligosacchrides. RBAs were heat stable up to 80 °C and stable at pH 4–10. RBA1 increased the transport of the fluorescent marker, rhodamine 123, which is known to be transported via the P-glycoprotein-mediated efflux pathway across human intestinal Caco-2 cell monolayers. Furthermore, RBA1 itself was transported to the basolateral side of the monolayers via an endocytotic pathway. View Full-Text
Keywords: Oryza sativa; lectin; rice bran lectin; Caco-2 cells Oryza sativa; lectin; rice bran lectin; Caco-2 cells
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Nakata, H.; Lin, C.Y.; Abolhassani, M.; Ogawa, T.; Tateno, H.; Hirabayashi, J.; Muramoto, K. Isolation of Rice Bran Lectins and Characterization of Their Unique Behavior in Caco-2 Cells. Int. J. Mol. Sci. 2017, 18, 1052.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top