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Int. J. Mol. Sci. 2017, 18(5), 1038; doi:10.3390/ijms18051038

The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo

1
Multidisciplinary Department of Medical-Surgical and Dental Specialties, Second University of Naples, Naples 80128, Italy
2
Department of Reconstructive Plastic Surgery-Hand Surgery, AOU “Ospedali Riuniti”, Ancona 60126, Italy
The authors contributed equally to these work.
*
Author to whom correspondence should be addressed.
Academic Editors: Adriano Piatelli and Yogesh Kumar Vashist
Received: 31 January 2017 / Revised: 26 March 2017 / Accepted: 28 April 2017 / Published: 12 May 2017
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Abstract

Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes. The results showed that in the middle-high density (MHD) layer there was a peak concentration of ASCs, compared to another layer obtained after centrifugation. Research carried out on patients under treatment for soft tissue regeneration using cells obtained from MHD layer selection will be fundamental in comparative analysis. These studies will lead to an adequate standardization of outcomes, provided that treatment is performed through cell selection. Therefore, a unique procedure in tissue reconstruction and regeneration through fat grafting is presented here. View Full-Text
Keywords: fat graft; adipose stem cells; regenerative surgery; breast reconstruction; pathological scar fat graft; adipose stem cells; regenerative surgery; breast reconstruction; pathological scar
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MDPI and ACS Style

De Francesco, F.; Guastafierro, A.; Nicoletti, G.; Razzano, S.; Riccio, M.; Ferraro, G.A. The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo. Int. J. Mol. Sci. 2017, 18, 1038.

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