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Int. J. Mol. Sci. 2017, 18(5), 0949; doi:10.3390/ijms18050949

Antiproliferative and Apoptotic Potential of Cyanidin-Based Anthocyanins on Melanoma Cells

Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, Victor Babeș Street 8, 400012 Cluj-Napoca, Romania
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
Author to whom correspondence should be addressed.
Academic Editors: Nuno Mateus and Iva Fernandes
Received: 14 February 2017 / Revised: 12 April 2017 / Accepted: 24 April 2017 / Published: 30 April 2017
(This article belongs to the Special Issue Anthocyanins)
View Full-Text   |   Download PDF [2839 KB, uploaded 30 April 2017]   |  


Elderberries are known for their high anthocyanins content, which have been shown to possess anti-proliferative and anti-cancer effects. Anthocyanins enriched extract (AEE) was obtained from elderberries and was characterized by LC/DAD/ESI-MS analysis. Five cyanidin-based anthocyanins were identified, among which Cy-3-O-samb was the major compound (51%). The total anthocyanins content of AEE was 495 mg Cy-3-O-samb/100 g FW. AEE inhibited proliferation of metastatic B16-F10 murine melanoma cells, in a concentration-dependent manner, with an IC50 of 264.3 μg/mL. LDH (lactate dehydrogenase), as a marker of membrane integrity, increased 74% in B16-F10 cells treated with 250 μg/mL AEE, compared to control. It was observed that apoptosis is the mechanism of melanoma cell death after AEE treatment, confirmed morphologically by acridine orange/ethidium bromide double staining and TUNEL analysis. These results indicate that elderberry-derived anthocyanins might be utilized in future applications as topical adjuvant in skin cancer therapy. View Full-Text
Keywords: anthocyanins; apoptosis; melanoma anthocyanins; apoptosis; melanoma

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Rugină, D.; Hanganu, D.; Diaconeasa, Z.; Tăbăran, F.; Coman, C.; Leopold, L.; Bunea, A.; Pintea, A. Antiproliferative and Apoptotic Potential of Cyanidin-Based Anthocyanins on Melanoma Cells. Int. J. Mol. Sci. 2017, 18, 0949.

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