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Int. J. Mol. Sci. 2017, 18(5), 0949; doi:10.3390/ijms18050949

Antiproliferative and Apoptotic Potential of Cyanidin-Based Anthocyanins on Melanoma Cells

1
Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
2
Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, Victor Babeș Street 8, 400012 Cluj-Napoca, Romania
3
Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
4
Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine, Mănăştur Street 3-5, 400372 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Academic Editors: Nuno Mateus and Iva Fernandes
Received: 14 February 2017 / Revised: 12 April 2017 / Accepted: 24 April 2017 / Published: 30 April 2017
(This article belongs to the Special Issue Anthocyanins)
View Full-Text   |   Download PDF [2839 KB, uploaded 30 April 2017]   |  

Abstract

Elderberries are known for their high anthocyanins content, which have been shown to possess anti-proliferative and anti-cancer effects. Anthocyanins enriched extract (AEE) was obtained from elderberries and was characterized by LC/DAD/ESI-MS analysis. Five cyanidin-based anthocyanins were identified, among which Cy-3-O-samb was the major compound (51%). The total anthocyanins content of AEE was 495 mg Cy-3-O-samb/100 g FW. AEE inhibited proliferation of metastatic B16-F10 murine melanoma cells, in a concentration-dependent manner, with an IC50 of 264.3 μg/mL. LDH (lactate dehydrogenase), as a marker of membrane integrity, increased 74% in B16-F10 cells treated with 250 μg/mL AEE, compared to control. It was observed that apoptosis is the mechanism of melanoma cell death after AEE treatment, confirmed morphologically by acridine orange/ethidium bromide double staining and TUNEL analysis. These results indicate that elderberry-derived anthocyanins might be utilized in future applications as topical adjuvant in skin cancer therapy. View Full-Text
Keywords: anthocyanins; apoptosis; melanoma anthocyanins; apoptosis; melanoma
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MDPI and ACS Style

Rugină, D.; Hanganu, D.; Diaconeasa, Z.; Tăbăran, F.; Coman, C.; Leopold, L.; Bunea, A.; Pintea, A. Antiproliferative and Apoptotic Potential of Cyanidin-Based Anthocyanins on Melanoma Cells. Int. J. Mol. Sci. 2017, 18, 0949.

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