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Int. J. Mol. Sci. 2017, 18(4), 720; doi:10.3390/ijms18040720

Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

1
Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain
2
Department of Agricultural Chemistry, Agrifood Campus of International Excellence ceiA3, University of Cordoba, 14014-Cordoba, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Martin Welch
Received: 10 February 2017 / Revised: 21 March 2017 / Accepted: 21 March 2017 / Published: 28 March 2017
(This article belongs to the Special Issue Biofilm Formation)
View Full-Text   |   Download PDF [2344 KB, uploaded 28 March 2017]   |  

Abstract

Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this study, we identify abundant proteins in the formation of the biofilm of an industrial flor yeast strain. A database search to enrich flor yeast “biological process” and “cellular component” according to Gene Ontology Terminology (GO Terms) and, “pathways” was carried out. The most abundant proteins detected were largely involved in respiration, translation, stress damage prevention and repair, amino acid metabolism (glycine, isoleucine, leucine and arginine), glycolysis/gluconeogenesis and biosynthesis of vitamin B9 (folate). These proteins were located in cellular components as in the peroxisome, mitochondria, vacuole, cell wall and extracellular region; being these two last directly related with the flor formation. Proteins like Bgl2p, Gcv3p, Hyp2p, Mdh1p, Suc2p and Ygp1p were quantified in very high levels. This study reveals some expected processes and provides new and important information for the design of conditions and genetic constructions of flor yeasts for improving the cellular survival and, thus, to optimize biological aging of Sherry wine production. View Full-Text
Keywords: Saccharomyces cerevisiae; flor velum; GO Terms; OFFGEL electrophoresis; LTQ Orbitrap XL MS Saccharomyces cerevisiae; flor velum; GO Terms; OFFGEL electrophoresis; LTQ Orbitrap XL MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Moreno-García, J.; Mauricio, J.C.; Moreno, J.; García-Martínez, T. Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation. Int. J. Mol. Sci. 2017, 18, 720.

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