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Int. J. Mol. Sci. 2017, 18(4), 170; doi:10.3390/ijms18040170

Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers

1
Clinical Laboratory, Faculdade de Medicina do ABC (FMABC), Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil
2
Biological Science Department, UNIFESP, Rua Prof. Artur Riedel, 275, CEP 09972-270 Diadema, SP, Brazil
3
Epidemiology Laboratory and Data Analysis, FMABC, Av. Príncipe de Gales, 821, CEP 09060-650 Santo André, SP, Brazil
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 27 September 2016 / Revised: 26 December 2016 / Accepted: 8 January 2017 / Published: 23 March 2017
(This article belongs to the Special Issue Liquid Biopsy for Clinical Application)
View Full-Text   |   Download PDF [672 KB, uploaded 23 March 2017]   |  

Abstract

Background: Patients with breast cancer—the deadliest cancer among women—are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value of MCT1, MCT4 and CD147 in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment. Methods: Differential expression of MCT1, MCT4 and CD147 obtained by qPCR was determined by 2−ΔΔCq method between biological samples (tumor and serial samples of peripheral) of patients (n = 125) and healthy women (n = 25). Results: tumor samples with higher histological grades have shown higher expression of these markers; this higher expression was also observed in blood samples obtained at diagnosis of patients when compared to healthy women and in patients with positive progression of the disease (metastasis development). Conclusion: markers studied here could be a promising strategy in routine laboratory evaluations as breast cancer diagnosis and prognosis. View Full-Text
Keywords: biomarkers; prognosis; metastasis; breast cancer; monocarboxylate transporter (MCT); hypoxia biomarkers; prognosis; metastasis; breast cancer; monocarboxylate transporter (MCT); hypoxia
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MDPI and ACS Style

Luz, M.C.B.; Perez, M.M.; Azzalis, L.A.; Sousa, L.V.A.; Adami, F.; Fonseca, F.L.A.; Alves, B.C.A. Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers. Int. J. Mol. Sci. 2017, 18, 170.

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