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Int. J. Mol. Sci. 2017, 18(2), 463; doi:10.3390/ijms18020463

Cryptomphalus aspersa Mollusc Egg Extract Promotes Regenerative Effects in Human Dermal Papilla Stem Cells

1
Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Darwin, 2, 28049 Madrid, Spain
2
Department of Pathology, Faculty of Medicine, Universidad de Málaga, Avda. Cervantes, 2, 29071 Málaga, Spain
3
Dermatology Service, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
4
Department of Medicine and Medical Specialties, Universidad de Alcalá, 28805 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Andrzej Slominski
Received: 7 December 2016 / Revised: 13 February 2017 / Accepted: 15 February 2017 / Published: 21 February 2017
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

The aim of this study was to test, by an in vitro approach, whether a natural extract derived from eggs of the mollusc Cryptomphalus aspersa (e-CAF) that seems to present regenerative properties, can enhance the mobilization of human hair dermal papilla cells (HHDPCs) and play a role on tissue repair and regeneration. We have tested HHDPCs proliferation by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium-bromide (MTT) assay; cell migration by using a wound healing assay, as well as the modulation of the expression of cytoskeletal (F-actin and vimentin) and cell adhesion to the extracellular matrix (ECM) (vinculin and P-FAK) proteins. We also explored whether e-CAF could lead HHDPCs to keratinocytes and/or fibroblasts by evaluating the expression of specific markers. We have compared these e-CAF effects with those induced by TGFβ1, implicated in regulation of cell proliferation and migration. e-CAF promotes proliferation and migration of HDDPCs cells in a time- and dose-dependent manner; it also increases the migratory behavior and the expression of adhesion molecules. These results support the fact that e-CAF could play a role on skin regeneration and be used for the prevention or repair of damaged tissue, either due to external causes or as a result of cutaneous aging. View Full-Text
Keywords: fibroblasts; proliferation; cell adhesion; cell differentiation; human dermal papilla cells; Cryptomphalus aspersa fibroblasts; proliferation; cell adhesion; cell differentiation; human dermal papilla cells; Cryptomphalus aspersa
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Alameda, M.T.; Morel, E.; Parrado, C.; González, S.; Juarranz, Á. Cryptomphalus aspersa Mollusc Egg Extract Promotes Regenerative Effects in Human Dermal Papilla Stem Cells. Int. J. Mol. Sci. 2017, 18, 463.

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