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Int. J. Mol. Sci. 2017, 18(2), 409; doi:10.3390/ijms18020409

The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b

1
Key Laboratory of Potato Biology and Biotechnology (Ministry of Agriculture, China), Huazhong Agricultural University, Wuhan 430070, China
2
School of Life Science, Yunnan Normal University, Kunming 650500, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 16 November 2016 / Revised: 3 February 2017 / Accepted: 6 February 2017 / Published: 14 February 2017
(This article belongs to the Section Molecular Botany)
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Abstract

Phytopathogenic oomycetes, such as Phytophthora infestans, potentially secrete many RxLR effector proteins into plant cells to modulate plant immune responses and promote colonization. However, the molecular mechanisms by which these RxLR effectors suppress plant immune responses are largely unknown. Here we describe an RxLR effector PITG_22798 (Gene accession: XM_002998349) that was upregulated during early infection of potato by P. infestans. By employment of agroinfiltration, we observed that PITG_22798 triggers cell death in Nicotiana benthamiana. Confocal microscopic examination showed that PITG_22798-GFP (Green Fluorescent Protein) located in the host nucleus when expressed transiently in N. benthamiana leaves. A nuclear localization signal (NLS) domain of PITG_22798 is important for nuclear localization and cell death-inducing activity. Sequence alignment and transient expression showed that PITG_22798 from diverse P. infestans isolates are conserved, and transient expression of PITG_22798 enhances P. infestans colonization of N. benthamiana leaves, which suggests that PITG_22798 contributes to P. infestans infection. PITG_22798-triggered cell death is dependent on SGT1-mediated signaling and is suppressed by the P. infestans avirulence effector 3b (AVR3b). The present research provides a clue for further investigation of how P. infestans effector PITG_22798 associates with and modulates host immunity. View Full-Text
Keywords: cell death; effector; Nicotiana benthamiana; Phytophthora infestans; plant immunity cell death; effector; Nicotiana benthamiana; Phytophthora infestans; plant immunity
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Wang, H.; Ren, Y.; Zhou, J.; Du, J.; Hou, J.; Jiang, R.; Wang, H.; Tian, Z.; Xie, C. The Cell Death Triggered by the Nuclear Localized RxLR Effector PITG_22798 from Phytophthora infestans Is Suppressed by the Effector AVR3b. Int. J. Mol. Sci. 2017, 18, 409.

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