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Int. J. Mol. Sci. 2017, 18(2), 362; doi:10.3390/ijms18020362

Localisation Microscopy of Breast Epithelial ErbB-2 Receptors and Gap Junctions: Trafficking after γ-Irradiation, Neuregulin-1β, and Trastuzumab Application

1
Kirchhoff Institute for Physics, Im Neuenheimer Feld INF 227, D-69120 Heidelberg, Germany
2
Bioquant-University of Heidelberg, Im Neuenheimer Feld INF 267, D-69120 Heidelberg, Germany
3
German Cancer Research Center, Im Neuenheimer Feld INF 280, D-69120 Heidelberg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Gregor Drummen
Received: 2 December 2016 / Revised: 28 January 2017 / Accepted: 30 January 2017 / Published: 9 February 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

In cancer, vulnerable breast epithelium malignance tendency correlates with number and activation of ErbB receptor tyrosine kinases. In the presented work, we observe ErbB receptors activated by irradiation-induced DNA injury or neuregulin- 1 β application, or alternatively, attenuated by a therapeutic antibody using high resolution fluorescence localization microscopy. The gap junction turnover coinciding with ErbB receptor activation and co-transport is simultaneously recorded. DNA injury caused by 4 Gray of 6 MeV photon γ -irradiation or alternatively neuregulin- 1 β application mobilized ErbB receptors in a nucleograde fashion—a process attenuated by trastuzumab antibody application. This was accompanied by increased receptor density, indicating packing into transport units. Factors mobilizing ErbB receptors also mobilized plasma membrane resident gap junction channels. The time course of ErbB receptor activation and gap junction mobilization recapitulates the time course of non-homologous end-joining DNA repair. We explain our findings under terms of DNA injury-induced membrane receptor tyrosine kinase activation and retrograde trafficking. In addition, we interpret the phenomenon of retrograde co-trafficking of gap junction connexons stimulated by ErbB receptor activation. View Full-Text
Keywords: breast cancer; ErbB receptor tyrosin kinase; gap junction; retrograde trafficking; irradiation; DNA injury; neuregulin; therapeutic antibody; spectral precision distance microscopy breast cancer; ErbB receptor tyrosin kinase; gap junction; retrograde trafficking; irradiation; DNA injury; neuregulin; therapeutic antibody; spectral precision distance microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pilarczyk, G.; Nesnidal, I.; Gunkel, M.; Bach, M.; Bestvater, F.; Hausmann, M. Localisation Microscopy of Breast Epithelial ErbB-2 Receptors and Gap Junctions: Trafficking after γ-Irradiation, Neuregulin-1β, and Trastuzumab Application. Int. J. Mol. Sci. 2017, 18, 362.

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