Next Article in Journal
Lung Infections in Systemic Rheumatic Disease: Focus on Opportunistic Infections
Next Article in Special Issue
Soy-Leaf Extract Exerts Atheroprotective Effects via Modulation of Krüppel-Like Factor 2 and Adhesion Molecules
Previous Article in Journal
Lannea coromandelica (Houtt.) Merr. Induces Heme Oxygenase 1 (HO-1) Expression and Reduces Oxidative Stress via the p38/c-Jun N-Terminal Kinase–Nuclear Factor Erythroid 2-Related Factor 2 (p38/JNK–NRF2)-Mediated Antioxidant Pathway
Previous Article in Special Issue
Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(2), 292; doi:10.3390/ijms18020292

Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model

1
MARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal
2
Marine Biodiscovery, School of Chemistry, National University of Ireland Galway, University Road, H91TK33 Galway, Ireland
The authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Leticia M. Estevinho
Received: 28 December 2016 / Revised: 19 January 2017 / Accepted: 24 January 2017 / Published: 29 January 2017
(This article belongs to the Special Issue Nutraceuticals in Human Health and Disease)
View Full-Text   |   Download PDF [1285 KB, uploaded 13 February 2017]   |  

Abstract

Antioxidants play an important role as Reactive Oxygen Species (ROS) chelating agents and, therefore, the screening for potent antioxidants from natural sources as potential protective agents is of great relevance. The main aim of this study was to obtain antioxidant-enriched fractions from the common seaweed Fucus spiralis and evaluate their activity and efficiency in protecting human cells (MCF-7 cells) on an oxidative stress condition induced by H2O2. Five fractions, F1–F5, were obtained by reversed-phase vacuum liquid chromatography. F3, F4 and F5 revealed the highest phlorotannin content, also showing the strongest antioxidant effects. The cell death induced by H2O2 was reduced by all fractions following the potency order F4 > F2 > F3 > F5 > F1. Only fraction F4 completely inhibited the H2O2 effect. To understand the possible mechanisms of action of these fractions, the cellular production of H2O2, the mitochondrial membrane potential and the caspase 9 activity were studied. Fractions F3 and F4 presented the highest reduction on H2O2 cell production. All fractions decreased both caspase-9 activity and cell membrane depolarization (except F1). Taken all together, the edible F. spiralis reveal that they provide protection against oxidative stress induced by H2O2 on the human MCF-7 cellular model, probably acting as upstream blockers of apoptosis. View Full-Text
Keywords: edible algae; oxidative stress; phlorotannins; marine natural products; MCF-7 cells; reactive oxygen species edible algae; oxidative stress; phlorotannins; marine natural products; MCF-7 cells; reactive oxygen species
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Pinteus, S.; Silva, J.; Alves, C.; Horta, A.; Thomas, O.P.; Pedrosa, R. Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model. Int. J. Mol. Sci. 2017, 18, 292.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top