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Int. J. Mol. Sci. 2017, 18(2), 292; doi:10.3390/ijms18020292

Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model

MARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, Portugal
Marine Biodiscovery, School of Chemistry, National University of Ireland Galway, University Road, H91TK33 Galway, Ireland
The authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Leticia M. Estevinho
Received: 28 December 2016 / Revised: 19 January 2017 / Accepted: 24 January 2017 / Published: 29 January 2017
(This article belongs to the Special Issue Nutraceuticals in Human Health and Disease)
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Antioxidants play an important role as Reactive Oxygen Species (ROS) chelating agents and, therefore, the screening for potent antioxidants from natural sources as potential protective agents is of great relevance. The main aim of this study was to obtain antioxidant-enriched fractions from the common seaweed Fucus spiralis and evaluate their activity and efficiency in protecting human cells (MCF-7 cells) on an oxidative stress condition induced by H2O2. Five fractions, F1–F5, were obtained by reversed-phase vacuum liquid chromatography. F3, F4 and F5 revealed the highest phlorotannin content, also showing the strongest antioxidant effects. The cell death induced by H2O2 was reduced by all fractions following the potency order F4 > F2 > F3 > F5 > F1. Only fraction F4 completely inhibited the H2O2 effect. To understand the possible mechanisms of action of these fractions, the cellular production of H2O2, the mitochondrial membrane potential and the caspase 9 activity were studied. Fractions F3 and F4 presented the highest reduction on H2O2 cell production. All fractions decreased both caspase-9 activity and cell membrane depolarization (except F1). Taken all together, the edible F. spiralis reveal that they provide protection against oxidative stress induced by H2O2 on the human MCF-7 cellular model, probably acting as upstream blockers of apoptosis. View Full-Text
Keywords: edible algae; oxidative stress; phlorotannins; marine natural products; MCF-7 cells; reactive oxygen species edible algae; oxidative stress; phlorotannins; marine natural products; MCF-7 cells; reactive oxygen species

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pinteus, S.; Silva, J.; Alves, C.; Horta, A.; Thomas, O.P.; Pedrosa, R. Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model. Int. J. Mol. Sci. 2017, 18, 292.

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