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Int. J. Mol. Sci. 2017, 18(2), 251; doi:10.3390/ijms18020251

Influence of Transgenic Metallothionein-1 on Gliosis, CA1 Neuronal Loss, and Brain Metal Levels of the Tg2576 Mouse Model of Alzheimer’s Disease

Department of Cellular Biology, Physiology and Immunology, and Institute of Neurosciences, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain
These authors contributed equally to this work.
Current address: Developmental Neurobiology and Regeneration Lab, Lab A1B1, Parc Científic de Barcelona, 08028 Barcelona, Spain.
*
Author to whom correspondence should be addressed.
Academic Editor: Masatoshi Maki
Received: 22 November 2016 / Revised: 17 January 2017 / Accepted: 19 January 2017 / Published: 26 January 2017
(This article belongs to the Special Issue Metalloproteins 2017)
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Abstract

The mouse model of Alzheimer’s disease (AD), Tg2576 mice (APP), has provided valuable information, such as the role of the metallothionein (MT) family in their behavioral and amyloidosis phenotypes. In this study, we further characterize the role of MT-1 by crossing Mt1-overexpressing mice with Tg2576 mice (APPTgMT). In 14-month-old mice, MT-1(/2) protein levels were dramatically increased by Mt1 overexpression throughout the cortex (Cx), which showed a prominent caudal-rostral gradient, and the hippocampus (HC). There was a trend for MT-1(/2) immunostaining to be increased in the areas surrounding the amyloid plaques in control male mice but not in Mt1-overexpressing mice. Gliosis was elicited by the amyloid plaques, but the effects of Mt1 overexpression were modest. However, in hippocampal western blots the microglial marker Iba-1 was increased in old male APPTgMT mice compared to APP-wild type (APPWT) mice, and the opposite was observed in young mice. Hippocampal CA1 neuronal loss was observed in Tg2576 mice, but was unaffected by Mt1 overexpression. Aging increased Zn and Cu levels differently depending on brain area, sex, and genotype. Thus, the effects of Mt1 overexpression on the phenotype of Tg2576 mice here studied are modest. View Full-Text
Keywords: Alzheimer’s disease; Tg2576; metallothionein-1; amyloid plaques; gliosis; CA1 neuronal loss; metals Alzheimer’s disease; Tg2576; metallothionein-1; amyloid plaques; gliosis; CA1 neuronal loss; metals
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MDPI and ACS Style

Comes, G.; Manso, Y.; Escrig, A.; Fernandez-Gayol, O.; Sanchis, P.; Molinero, A.; Giralt, M.; Carrasco, J.; Hidalgo, J. Influence of Transgenic Metallothionein-1 on Gliosis, CA1 Neuronal Loss, and Brain Metal Levels of the Tg2576 Mouse Model of Alzheimer’s Disease. Int. J. Mol. Sci. 2017, 18, 251.

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