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Int. J. Mol. Sci. 2017, 18(12), 2768; doi:10.3390/ijms18122768

Genome-Wide Identification, Evolutionary and Expression Analyses of the GALACTINOL SYNTHASE Gene Family in Rapeseed and Tobacco

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1,* , 3,4,* and 1,2,*
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College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
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Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
3
Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Academy of Tobacco Science, Guiyang 550081, China
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Upland Flue-Cured Tobacco Quality and Ecology Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang 550081, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 30 September 2017 / Revised: 16 November 2017 / Accepted: 17 December 2017 / Published: 20 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Galactinol synthase (GolS) is a key enzyme in raffinose family oligosaccharide (RFO) biosynthesis. The finding that GolS accumulates in plants exposed to abiotic stresses indicates RFOs function in environmental adaptation. However, the evolutionary relationships and biological functions of GolS family in rapeseed (Brassica napus) and tobacco (Nicotiana tabacum) remain unclear. In this study, we identified 20 BnGolS and 9 NtGolS genes. Subcellular localization predictions showed that most of the proteins are localized to the cytoplasm. Phylogenetic analysis identified a lost event of an ancient GolS copy in the Solanaceae and an ancient duplication event leading to evolution of GolS4/7 in the Brassicaceae. The three-dimensional structures of two GolS proteins were conserved, with an important DxD motif for binding to UDP-galactose (uridine diphosphate-galactose) and inositol. Expression profile analysis indicated that BnGolS and NtGolS genes were expressed in most tissues and highly expressed in one or two specific tissues. Hormone treatments strongly induced the expression of most BnGolS genes and homologous genes in the same subfamilies exhibited divergent-induced expression. Our study provides a comprehensive evolutionary analysis of GolS genes among the Brassicaceae and Solanaceae as well as an insight into the biological function of GolS genes in hormone response in plants. View Full-Text
Keywords: galactinol synthase; Brassica napus; Nicotiana tabacum; evolution; expression galactinol synthase; Brassica napus; Nicotiana tabacum; evolution; expression
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Fan, Y.; Yu, M.; Liu, M.; Zhang, R.; Sun, W.; Qian, M.; Duan, H.; Chang, W.; Ma, J.; Qu, C.; Zhang, K.; Lei, B.; Lu, K. Genome-Wide Identification, Evolutionary and Expression Analyses of the GALACTINOL SYNTHASE Gene Family in Rapeseed and Tobacco. Int. J. Mol. Sci. 2017, 18, 2768.

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