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Int. J. Mol. Sci. 2017, 18(12), 2676; doi:10.3390/ijms18122676

miR-34a Regulates Sperm Motility in Zebrafish

1
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China
2
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan 430072, China
*
Author to whom correspondence should be addressed.
Received: 25 October 2017 / Revised: 29 November 2017 / Accepted: 7 December 2017 / Published: 10 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system. miR-34a knockout zebrafish showed no obvious defects on testis morphology and sperm quantity. However, we found a significant increase in progressive sperm motility that is one of the pivotal factors influencing in vitro fertilization rates, in the knockout zebrafish. Moreover, breeding experiments showed that, when miR-34a-knockout male zebrafish mated with the wide-type females, they had a higher fertilization rate than did the wide-type males. Glycogen synthase kinase-3a (gsk3a), a potential sperm motility regulatory gene was predicted to be targeted by miR-34a, which was further supported by luciferase reporter assays, since a significant decrease of luciferase activity was detected upon ectopic overexpression of miR-34a. Our findings suggest that miR-34a downregulates gsk3a by targeting its 3′ untranslated region, and miR-34a/gsk3a interaction modulates sperm motility in zebrafish. This study will help in understanding in the role of the miR-34 family during spermatogenesis and will set paths for further studies. View Full-Text
Keywords: miR-34a; knockout; sperm motility; gsk3a miR-34a; knockout; sperm motility; gsk3a
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Guo, W.; Xie, B.; Xiong, S.; Liang, X.; Gui, J.-F.; Mei, J. miR-34a Regulates Sperm Motility in Zebrafish. Int. J. Mol. Sci. 2017, 18, 2676.

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