miR-34a Regulates Sperm Motility in Zebrafish
AbstractIncreasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system. miR-34a knockout zebrafish showed no obvious defects on testis morphology and sperm quantity. However, we found a significant increase in progressive sperm motility that is one of the pivotal factors influencing in vitro fertilization rates, in the knockout zebrafish. Moreover, breeding experiments showed that, when miR-34a-knockout male zebrafish mated with the wide-type females, they had a higher fertilization rate than did the wide-type males. Glycogen synthase kinase-3a (gsk3a), a potential sperm motility regulatory gene was predicted to be targeted by miR-34a, which was further supported by luciferase reporter assays, since a significant decrease of luciferase activity was detected upon ectopic overexpression of miR-34a. Our findings suggest that miR-34a downregulates gsk3a by targeting its 3′ untranslated region, and miR-34a/gsk3a interaction modulates sperm motility in zebrafish. This study will help in understanding in the role of the miR-34 family during spermatogenesis and will set paths for further studies. View Full-Text
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Guo, W.; Xie, B.; Xiong, S.; Liang, X.; Gui, J.-F.; Mei, J. miR-34a Regulates Sperm Motility in Zebrafish. Int. J. Mol. Sci. 2017, 18, 2676.
Guo W, Xie B, Xiong S, Liang X, Gui J-F, Mei J. miR-34a Regulates Sperm Motility in Zebrafish. International Journal of Molecular Sciences. 2017; 18(12):2676.Chicago/Turabian Style
Guo, Wenjie; Xie, Binyue; Xiong, Shuting; Liang, Xufang; Gui, Jian-Fang; Mei, Jie. 2017. "miR-34a Regulates Sperm Motility in Zebrafish." Int. J. Mol. Sci. 18, no. 12: 2676.
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