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Int. J. Mol. Sci. 2017, 18(12), 2635; doi:10.3390/ijms18122635

Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway

1
Department of Oral Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Biotooth Regeneration, Kyungpook National University, Daegu 41940, Korea
2
Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
3
Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan 47227, Korea
*
Author to whom correspondence should be addressed.
Received: 2 November 2017 / Revised: 1 December 2017 / Accepted: 4 December 2017 / Published: 6 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Marine algae possess a variety of beneficial effects on human health. In this study, we investigated whether diphlorethohydroxycarmalol (DPHC), isolated from Ishige okamurae, a brown alga, suppresses receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. DPHC significantly suppressed RANKL-induced osteoclast differentiation and macrophage-colony stimulating factor (M-CSF) expression in a dose-dependent manner. In addition, it significantly inhibited actin ring formation, the expression of osteoclast marker genes, such as tartrate-resistant acid phosphatase (TRAP), nuclear factor of activated T-cells cytoplasmic 1 (Nfatc1), cathepsin K (Ctsk), and dendritic cell-specific transmembrane protein (Dcstamp), and osteoclast-induced bone resorption. Analysis of the RANKL-mediated signaling pathway showed that the phosphorylation of both IκB and p65 was specifically inhibited by DPHC. These results suggest that DPHC substantially suppresses osteoclastogenesis by downregulating the RANK-NF-κB signaling pathway. Thus, it holds significant potential for the treatment of skeletal diseases associated with an enhanced osteoclast activity. View Full-Text
Keywords: diphlorethohydroxycarmalol; brown algae; osteoclast; NF-κB diphlorethohydroxycarmalol; brown algae; osteoclast; NF-κB
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MDPI and ACS Style

Ihn, H.J.; Kim, J.A.; Cho, H.S.; Shin, H.-I.; Kim, G.-Y.; Choi, Y.H.; Jeon, Y.-J.; Park, E.K. Diphlorethohydroxycarmalol from Ishige okamurae Suppresses Osteoclast Differentiation by Downregulating the NF-κB Signaling Pathway. Int. J. Mol. Sci. 2017, 18, 2635.

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