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Int. J. Mol. Sci. 2017, 18(12), 2572; doi:10.3390/ijms18122572

Structural Masquerade of Plesiomonas shigelloides Strain CNCTC 78/89 O-Antigen—High-Resolution Magic Angle Spinning NMR Reveals the Modified d-galactan I of Klebsiella pneumoniae

Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
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Received: 8 November 2017 / Revised: 23 November 2017 / Accepted: 25 November 2017 / Published: 29 November 2017
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
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Abstract

The high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR) analysis of Plesiomonas shigelloides 78/89 lipopolysaccharide directly on bacteria revealed the characteristic structural features of the O-acetylated polysaccharide in the NMR spectra. The O-antigen profiles were unique, yet the pattern of signals in the, spectra along with their 1H,13C chemical shift values, resembled these of d-galactan I of Klebsiella pneumoniae. The isolated O-specific polysaccharide (O-PS) of P. shigelloides strain CNCTC 78/89 was investigated by 1H and 13C NMR spectroscopy, mass spectrometry and chemical methods. The analyses demonstrated that the P. shigelloides 78/89 O-PS is composed of →3)-α-d-Galp-(1→3)-β-d-Galf2OAc-(1→ disaccharide repeating units. The O-acetylation was incomplete and resulted in a microheterogeneity of the O-antigen. This O-acetylation generates additional antigenic determinants within the O-antigen, forms a new chemotype, and contributes to the epitopes recognized by the O-serotype specific antibodies. The serological cross-reactivities further confirmed the inter-specific structural similarity of these O-antigens. View Full-Text
Keywords: Plesiomonas shigelloides; O-antigen; lipopolysaccharide; O-acetylation; d-galactan I; HR-MAS; NMR spectroscopy Plesiomonas shigelloides; O-antigen; lipopolysaccharide; O-acetylation; d-galactan I; HR-MAS; NMR spectroscopy
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Ucieklak, K.; Koj, S.; Pawelczyk, D.; Niedziela, T. Structural Masquerade of Plesiomonas shigelloides Strain CNCTC 78/89 O-Antigen—High-Resolution Magic Angle Spinning NMR Reveals the Modified d-galactan I of Klebsiella pneumoniae. Int. J. Mol. Sci. 2017, 18, 2572.

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