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Int. J. Mol. Sci. 2017, 18(11), 2446; https://doi.org/10.3390/ijms18112446

Molecular Alliance of Lymantria dispar Multiple Nucleopolyhedrovirus and a Short Unmodified Antisense Oligonucleotide of Its Anti-Apoptotic IAP-3 Gene: A Novel Approach for Gypsy Moth Control

1
Taurida Academy, Department of Biochemistry, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Republic of Crimea
2
Medical Academy, Department of Biochemistry, V.I. Vernadsky Crimean Federal University, Simferopol 295006, Republic of Crimea
3
Medical Academy, Department of Pathological Anatomy, V.I. Vernadsky Crimean Federal University, Simferopol 295006, Republic of Crimea
4
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia
5
Department of Virology, Lomonosov Moscow State University, Moscow 119991, Russia
6
Center of Bioengineering, Russian Academy of Sciences, Moscow 117312, Russia
7
Translational Biomedicine Laboratory, N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow 123098, Russia
8
Institute of Natural Sciences, Ural Federal University, Chelyabinsk 620083, Russia
9
Medical Academy, Department of General and Clinical Pathophysiology, V.I. Vernadsky Crimean Federal University, Simferopol 295006, Republic of Crimea
10
Department of Polymer Chemistry, Mendeleev University of Chemical Technology of Russia, Moscow 125047, Russia
11
University of Torino, 10124 Torino, Italy
12
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 10691 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Received: 20 October 2017 / Revised: 11 November 2017 / Accepted: 15 November 2017 / Published: 17 November 2017
(This article belongs to the Special Issue Molecular Entomology of Insects of Economic Importance)
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Abstract

Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides. View Full-Text
Keywords: baculovirus infection; gypsy moth Lymantria dispar; multiple nucleopolyhedrovirus IAP (inhibitor-of-apoptosis) genes; RING (really interesting new gene); DNA insecticides; insect pest management baculovirus infection; gypsy moth Lymantria dispar; multiple nucleopolyhedrovirus IAP (inhibitor-of-apoptosis) genes; RING (really interesting new gene); DNA insecticides; insect pest management
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Oberemok, V.V.; Laikova, K.V.; Zaitsev, A.S.; Shumskykh, M.N.; Kasich, I.N.; Gal’chinsky, N.V.; Bekirova, V.V.; Makarov, V.V.; Agranovsky, A.A.; Gushchin, V.A.; Zubarev, I.V.; Kubyshkin, A.V.; Fomochkina, I.I.; Gorlov, M.V.; Skorokhod, O.A. Molecular Alliance of Lymantria dispar Multiple Nucleopolyhedrovirus and a Short Unmodified Antisense Oligonucleotide of Its Anti-Apoptotic IAP-3 Gene: A Novel Approach for Gypsy Moth Control. Int. J. Mol. Sci. 2017, 18, 2446.

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