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Int. J. Mol. Sci. 2017, 18(11), 2444; https://doi.org/10.3390/ijms18112444

Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System

1
College of Plant Sciences, Jilin University, Changchun 130062, China
2
College of Resource and Environment, Jilin Agricultural University, Changchun 130062, China
*
Authors to whom correspondence should be addressed.
Received: 11 October 2017 / Revised: 13 November 2017 / Accepted: 15 November 2017 / Published: 17 November 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Plant productivity is limited by salinity stress, both in natural and agricultural systems. Identification of salt stress-related genes from halophyte can provide insights into mechanisms of salt stress tolerance in plants. Atriplex canescens is a xero-halophyte that exhibits optimum growth in the presence of 400 mM NaCl. A cDNA library derived from highly salt-treated A. canescens plants was constructed based on a yeast expression system. A total of 53 transgenic yeast clones expressing enhanced salt tolerance were selected from 105 transformants. Their plasmids were sequenced and the gene characteristics were annotated using a BLASTX search. Retransformation of yeast cells with the selected plasmids conferred salt tolerance to the resulting transformants. The expression patterns of 28 of these stress-related genes were further investigated in A. canescens leaves by quantitative reverse transcription-PCR. In this study, we provided a rapid and robust assay system for large-scale screening of genes for varied abiotic stress tolerance with high efficiency in A. canescens. View Full-Text
Keywords: Atriplex canescens; salinity tolerance; yeast expression; halophyte; Gene Othology analysis Atriplex canescens; salinity tolerance; yeast expression; halophyte; Gene Othology analysis
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Yu, G.; Li, J.; Sun, X.; Liu, Y.; Wang, X.; Zhang, H.; Pan, H. Exploration for the Salinity Tolerance-Related Genes from Xero-Halophyte Atriplex canescens Exploiting Yeast Functional Screening System. Int. J. Mol. Sci. 2017, 18, 2444.

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