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Int. J. Mol. Sci. 2017, 18(11), 2428; https://doi.org/10.3390/ijms18112428

Analysis of the Roles of the Arabidopsis nMAT2 and PMH2 Proteins Provided with New Insights into the Regulation of Group II Intron Splicing in Land-Plant Mitochondria

1
Department of Plant and Environmental Sciences, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat-Ram, Jerusalem 91904, Israel
2
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794, USA
*
Author to whom correspondence should be addressed.
Received: 28 September 2017 / Revised: 5 November 2017 / Accepted: 7 November 2017 / Published: 17 November 2017
(This article belongs to the Special Issue Plant Mitochondria)
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Abstract

Plant mitochondria are remarkable with respect to the presence of numerous group II introns which reside in many essential genes. The removal of the organellar introns from the coding genes they interrupt is essential for respiratory functions, and is facilitated by different enzymes that belong to a diverse set of protein families. These include maturases and RNA helicases related proteins that function in group II intron splicing in different organisms. Previous studies indicate a role for the nMAT2 maturase and the RNA helicase PMH2 in the maturation of different pre-RNAs in Arabidopsis mitochondria. However, the specific roles of these proteins in the splicing activity still need to be resolved. Using transcriptome analyses of Arabidopsis mitochondria, we show that nMAT2 and PMH2 function in the splicing of similar subsets of group II introns. Fractionation of native organellar extracts and pulldown experiments indicate that nMAT2 and PMH2 are associated together with their intron-RNA targets in large ribonucleoprotein particle in vivo. Moreover, the splicing efficiencies of the joint intron targets of nMAT2 and PMH2 are more strongly affected in a double nmat2/pmh2 mutant-line. These results are significant as they may imply that these proteins serve as components of a proto-spliceosomal complex in plant mitochondria. View Full-Text
Keywords: group II introns; splicing; maturases; RNA helicases; mitochondria; Arabidopsis; angiosperms group II introns; splicing; maturases; RNA helicases; mitochondria; Arabidopsis; angiosperms
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Zmudjak, M.; Shevtsov, S.; Sultan, L.D.; Keren, I.; Ostersetzer-Biran, O. Analysis of the Roles of the Arabidopsis nMAT2 and PMH2 Proteins Provided with New Insights into the Regulation of Group II Intron Splicing in Land-Plant Mitochondria. Int. J. Mol. Sci. 2017, 18, 2428.

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