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Int. J. Mol. Sci. 2017, 18(11), 2391; https://doi.org/10.3390/ijms18112391

Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates

1
Centro/Departamento de Física, Universidade do Minho, 4710-057 Braga, Portugal
2
Centre for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València, 46022 Valencia, Spain
3
Hematology Research Group, Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain
4
Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Instituto Carlos III, 28029 Madrid, Spain
5
Hematology Department, Hospital Universitario y Politécnico La Fe, 46026 Valencia, Spain
6
Centro/Departamento de Química, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal
7
CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
8
BCMaterials, Parque Científico y Tecnológico de Bizkaia, 48160 Derio, Spain
9
IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain
10
Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 29 September 2017 / Revised: 6 November 2017 / Accepted: 9 November 2017 / Published: 11 November 2017
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
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Abstract

The aim of this work was to determine the influence of the biomaterial environment on human mesenchymal stem cell (hMSC) fate when cultured in supports with varying topography. Poly(vinylidene fluoride) (PVDF) culture supports were prepared with structures ranging between 2D and 3D, based on PVDF films on which PVDF microspheres were deposited with varying surface density. Maintenance of multipotentiality when cultured in expansion medium was studied by flow cytometry monitoring the expression of characteristic hMSCs markers, and revealed that cells were losing their characteristic surface markers on these supports. Cell morphology was assessed by scanning electron microscopy (SEM). Alkaline phosphatase activity was also assessed after seven days of culture on expansion medium. On the other hand, osteoblastic differentiation was monitored while culturing in osteogenic medium after cells reached confluence. Osteocalcin immunocytochemistry and alizarin red assays were performed. We show that flow cytometry is a suitable technique for the study of the differentiation of hMSC seeded onto biomaterials, giving a quantitative reliable analysis of hMSC-associated markers. We also show that electrosprayed piezoelectric poly(vinylidene fluoride) is a suitable support for tissue engineering purposes, as hMSCs can proliferate, be viable and undergo osteogenic differentiation when chemically stimulated. View Full-Text
Keywords: tissue engineering; bone differentiation; poly(vinylidene fluoride); microspheres tissue engineering; bone differentiation; poly(vinylidene fluoride); microspheres
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sobreiro-Almeida, R.; Tamaño-Machiavello, M.N.; Carvalho, E.O.; Cordón, L.; Doria, S.; Senent, L.; Correia, D.M.; Ribeiro, C.; Lanceros-Méndez, S.; Sabater i Serra, R.; Gomez Ribelles, J.L.; Sempere, A. Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates. Int. J. Mol. Sci. 2017, 18, 2391.

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