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Int. J. Mol. Sci. 2017, 18(11), 2348; https://doi.org/10.3390/ijms18112348

Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

1
Research Institute, Femtobiomed Co., Ltd., 700, Pangyo-ro, Seongnam-si, Gyeonggi-do 13516, Korea
2
Department of Mechanical System Engineering, Korea Polytechnic Univsersity, 237 Sangidaehak-ro, Siheung-si, Gyoenggi-do 15073, Korea
3
Department of Advanced Convergence Technology, Korea Polytechnic Univsersity, 237 Sangidaehak-ro, Siheung-si, Gyoenggi-do 15073, Korea
4
Research Institute, T&R Biofab Co., Ltd., 237 Sangidaehak-ro, Siheung-si, Gyoenggi-do 15073, Korea
5
Department of Mechanical Engineering, Korea Polytechnic Univsersity, 237 Sangidaehak-ro, Siheung-si, Gyoenggi-do 15073, Korea
6
School of Mechanical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, Korea
7
Department of Pharmacology, Ajou University School of Medicine, Suwon 442-721, Korea
8
Department of Anatomy, Pusan National University School of Medicine, Yangsan 626-770, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 21 September 2017 / Revised: 29 October 2017 / Accepted: 2 November 2017 / Published: 7 November 2017
(This article belongs to the Special Issue Nano/Micro-Assisted Regenerative Medicine)
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Abstract

In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies. View Full-Text
Keywords: in vitro cancer model; inkjet cell printing; microtissue array; cancer drug discovery; electrospinning; nanofibrous membrane in vitro cancer model; inkjet cell printing; microtissue array; cancer drug discovery; electrospinning; nanofibrous membrane
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Park, T.-M.; Kang, D.; Jang, I.; Yun, W.-S.; Shim, J.-H.; Jeong, Y.H.; Kwak, J.-Y.; Yoon, S.; Jin, S. Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing. Int. J. Mol. Sci. 2017, 18, 2348.

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