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Int. J. Mol. Sci. 2017, 18(11), 2324; doi:10.3390/ijms18112324

Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production

Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan City 32003, Taiwan
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin City 64002, Taiwan
Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung City 81157, Taiwan
Author to whom correspondence should be addressed.
Received: 10 October 2017 / Revised: 26 October 2017 / Accepted: 31 October 2017 / Published: 3 November 2017
(This article belongs to the Section Bioactives and Nutraceuticals)
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Hydrogels of 2-hydroxyethyl methacrylate/polyethylene glycol diacrylate (HEMA/PEGDA) have been extensively studied for their use in biomedical and pharmaceutical applications owing to their nontoxic and highly hydrophilic characteristics. Recently, cells immobilized by HEMA/PEGDA hydrogels have also been studied for enhanced production in fermentation. Hydrogel films of HEMA/PEGDA copolymer were generated by Ultraviolet (UV)-initiated photopolymerization. The hydrogel films were used to immobilize viable Lactobacillus brevis RK03 cells for the bioconversion of monosodium glutamate (MSG) to γ-aminobutyric acid (GABA). The mechanical properties and fermentation yields of the L. brevis RK03 cells immobilized on polyacrylate hydrogel films with different monomeric formulations were investigated. Fermentation was carried out in 75 mL de Man, Rogosa and Sharpe (MRS) medium containing various concentrations of MSG. We found that HEMA (93%)/PEGDA (3%) hydrogels (sample H) maximized GABA production. The conversion rate of MSG to GABA reached a maximum value of 98.4% after 240 h. Bioconversion activity gradually declined after 420 h to 83.8% after five cycles of semi-continuous fermentation. Our results suggest that HEMA (93%)/PEGDA (3%) hydrogels have great potential for use in GABA production via semi-continuous fermentation. View Full-Text
Keywords: hydrogel; hydroxyethyl methacrylate; polyethylene glycol diacrylate; gamma aminobutyric acid; immobilized; Lactobacillus brevis hydrogel; hydroxyethyl methacrylate; polyethylene glycol diacrylate; gamma aminobutyric acid; immobilized; Lactobacillus brevis

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hsueh, Y.-H.; Liaw, W.-C.; Kuo, J.-M.; Deng, C.-S.; Wu, C.-H. Hydrogel Film-Immobilized Lactobacillus brevis RK03 for γ-Aminobutyric Acid Production. Int. J. Mol. Sci. 2017, 18, 2324.

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