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Int. J. Mol. Sci. 2017, 18(11), 2320; https://doi.org/10.3390/ijms18112320

Low Temperature Extends the Lifespan of Bursaphelenchus xylophilus through the cGMP Pathway

1
College of Forestry, Northeast Forestry University, Harbin 150040, China
2
College of Management, Harbin University of Commerce, Harbin 150028, China
*
Authors to whom correspondence should be addressed.
Received: 27 August 2017 / Revised: 18 October 2017 / Accepted: 30 October 2017 / Published: 3 November 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The causal agent of pine wilt disease, pine wood nematode (PWN) (Bursaphelenchus xylophilus), revealed extended lifespan at low temperature. To discover the molecular mechanism of this phenomenon, we attempted to study the molecular characterization, transcript abundance, and functions of three genes of the cyclic guanosine monophosphate (cGMP) pathway from B. xylophilus. Three cGMP pathway genes were identified from B. xylophilus. Bioinformatic software was utilized to analyze the characteristics of the three putative proteins. Function of the three genes in cold tolerance was studied with RNA interference (RNAi). The results showed that the deduced protein of Bx-DAF-11 has an adenylate and guanylate cyclase catalytic domain, indicating an ability to bind to extracellular ligands and synthesizing cGMP. Both Bx-TAX-2 and Bx-TAX-4 have cyclic nucleotide-binding domains and ion transport protein domains, illustrating that they are cGMP-gated ion channels. The transcript level of Bx-daf-11, Bx-tax-2, and Bx-tax-4 increased at low temperature. The survival rates of three gene silenced B. xylophilus revealed a significant decrease at low temperature. This study illustrated that the cGMP pathway plays a key role in low-temperature-induced lifespan extension in B. xylophilus. View Full-Text
Keywords: Bursaphelenchus xylophilus; pine wilt disease; cGMP pathway; low temperature; lifespan extension Bursaphelenchus xylophilus; pine wilt disease; cGMP pathway; low temperature; lifespan extension
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, B.; Ma, L.; Wang, F.; Wang, B.; Hao, X.; Xu, J.; Ma, Y. Low Temperature Extends the Lifespan of Bursaphelenchus xylophilus through the cGMP Pathway. Int. J. Mol. Sci. 2017, 18, 2320.

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