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Int. J. Mol. Sci. 2017, 18(11), 2286; https://doi.org/10.3390/ijms18112286

Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas

Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Tokyo 113-8657, Japan
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Received: 30 September 2017 / Revised: 26 October 2017 / Accepted: 26 October 2017 / Published: 31 October 2017
(This article belongs to the Special Issue Genome Editing 2018)
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Abstract

Mammalian zygote-mediated genome-engineering by Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas is currently used for the generation of genome-modified animals. Here, we report that a Campylobacter jejuni-derived orthologous CRISPR/Cas system recognizes a 5′-NNNVRYAC sequence as a protospacer-adjacent motif in mouse zygotes, and is applicable for efficient generation of knockout mice. Moreover, this novel CRISPR/Cas can be used for zygote-mediated knock-in at a unique locus, suggesting that this system could help to expand the feasibility of the zygote-mediated generation of genome-modified animals. View Full-Text
Keywords: CRISPR/Cas; genome-modified mouse; Campylobacter jejuni CRISPR/Cas; genome-modified mouse; Campylobacter jejuni
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fujii, W.; Ikeda, A.; Sugiura, K.; Naito, K. Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas. Int. J. Mol. Sci. 2017, 18, 2286.

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