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Int. J. Mol. Sci. 2017, 18(11), 2278; https://doi.org/10.3390/ijms18112278

Identification of Two Cytochrome Monooxygenase P450 Genes, CYP321A7 and CYP321A9, from the Tobacco Cutworm Moth (Spodoptera Litura) and Their Expression in Response to Plant Allelochemicals

1
Guangdong Engineering Research Center for Modern Eco-Agruculture and Circular Agriculture, Guangzhou 510642, China
2
Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China
3
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, East Campus, Guangzhou 510006, China
4
Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Received: 6 September 2017 / Revised: 13 October 2017 / Accepted: 27 October 2017 / Published: 30 October 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Larvae of the polyphagous tobacco cutworm moth, Spodoptera litura (S. litura), encounter potentially toxic allelochemicals in food. It is therefore important for S. litura to produce detoxification enzymes such as cytochrome P450 monooxygenases (P450s). In this study, we have identified two novel cytochrome P450 genes of S. litura, named CYP321A7 and CYP321A9. Phylogenetic analysis indicated that they belong to the CYP321A subfamily. Expression levels of these genes at different development stages were determined by real-time quantitative polymerase chain reaction (PCR). The highest expression was found in the midgut and the fat body. Larvae fed with a diet supplemented with xanthotoxin or coumarin showed a strongly increased expression of CYP321A7 and CYP321A9 in the midgut and fat body as compared to larvae that consumed a control diet. In contrast, larvae consuming a diet containing aflatoxin B1 or quercetin did not induce the expression of these genes. CYP321A7 and CYP321A9 showed different expression profiles with respect to certain allelochemicals. For example, a diet containing cinnamic acid stimulated the expression of CYP321A9, whereas no changes were observed for CYP321A7. We suggest that the fine tuning of P450 gene expression is an important adaptation mechanism that allows polyphagous S. litura larvae to survive in a changing chemical environment. View Full-Text
Keywords: cytochrome P450 monooxygenases; Spodoptera litura; plant allelochemicals; CYP321A7; CYP321A9 cytochrome P450 monooxygenases; Spodoptera litura; plant allelochemicals; CYP321A7; CYP321A9
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Wang, R.-L.; He, Y.-N.; Staehelin, C.; Liu, S.-W.; Su, Y.-J.; Zhang, J.-E. Identification of Two Cytochrome Monooxygenase P450 Genes, CYP321A7 and CYP321A9, from the Tobacco Cutworm Moth (Spodoptera Litura) and Their Expression in Response to Plant Allelochemicals. Int. J. Mol. Sci. 2017, 18, 2278.

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