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Int. J. Mol. Sci. 2017, 18(11), 2248; https://doi.org/10.3390/ijms18112248

Aspidosperma pyrifolium Has Anti-Inflammatory Properties: An Experimental Study in Mice with Peritonitis Induced by Tityus serrulatus Venom or Carrageenan

1
Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil
2
Laboratory of Pharmacognosy (PNBio), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil
3
Laboratory of Biotechnology of Natural Biopolymers, Department of Biochemistry, Bioscience Center, Campus Universitário, Federal University of Rio Grande do Norte, Avenida Senador Salgado Filho, 3000, Lagoa Nova 59072-970, Natal, Brazil
4
Laboratory of Pathology, Departament of Pathology, Federal University of Rio Grande do Norte, Rua General Gustavo Cordeiro de Farias, S/N, Petrópolis 59012-570, Natal, Brazil
*
Author to whom correspondence should be addressed.
Received: 15 September 2017 / Revised: 27 September 2017 / Accepted: 20 October 2017 / Published: 11 November 2017
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols 2018)
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Abstract

Scorpions of the genus Tityus are responsible for the majority of envenomation in Brazil, the Tityus serrulatus species being the most common and dangerous in South America. In this approach, we have investigated the ability of the aqueous extract from the leaves of Aspidosperma pyrifolium in reducing carrageenan-induced inflammation and the inflammation induced by T. serrulatus envenomation in mice. We also evaluated the cytotoxic effects of this extract, using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-2H-tetrazolium (MTT) assay and the results revealed that the extract is safe. Analysis by High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD) and Liquid Chromatography Coupled with Mass Spectrometry with Diode Array Detection (LC-DAD-MS) showed one major chemical component, the flavonoid rutin and phenolics compounds. For in vivo studies in carrageenan-induced peritonitis model, mice received extracts, dexamethasone, rutin or saline, before administration of carrageenan. For venom-induced inflammation model, animals received T. serrulatus venom and were, simultaneously, treated with extracts, antivenom, rutin or saline. The extract and rutin showed a reduction in the cell migration into the peritoneal cavity, and in the same way the envenomated animals also showed reduction of edema, inflammatory cell infiltration and vasodilation in lungs. This is an original study revealing the potential action of A. pyrifolium against inflammation caused by Tityus serrulatus venom and carrageenan, revealing that this extract and its bioactive molecules, specifically rutin, may present potential anti-inflammatory application. View Full-Text
Keywords: anti-inflammatory activity; Aspidosperma pyrifolium; carrageenan; rutin; scorpion venom; Tityus serrulatus anti-inflammatory activity; Aspidosperma pyrifolium; carrageenan; rutin; scorpion venom; Tityus serrulatus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Souza Lima, M.C.J.; Oliveira Bitencourt, M.A.; Furtado, A.A.; Torres-Rêgo, M.; Siqueira, E.M.S.; Oliveira, R.M.; Oliveira Rocha, H.A.; Ferreira Rocha, K.B.; Silva-Júnior, A.A.; Zucolotto, S.M.; Fernandes-Pedrosa, M.F. Aspidosperma pyrifolium Has Anti-Inflammatory Properties: An Experimental Study in Mice with Peritonitis Induced by Tityus serrulatus Venom or Carrageenan. Int. J. Mol. Sci. 2017, 18, 2248.

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