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Int. J. Mol. Sci. 2017, 18(10), 2139; https://doi.org/10.3390/ijms18102139

Bone Marrow-Derived Stem Cell Populations Are Differentially Regulated by Thyroid or/and Ovarian Hormone Loss

1
Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculty, Ave. Silva Jardim 1632, Box 80.250-200 Curitiba, Paraná, Brazil
2
Feinberg School of Medicine, Feinberg Cardiovascular Research Institute, Northwestern University, 303 E. Chicago Ave., Tarry 14-725, Chicago, IL 60611, USA
3
Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição, 1155, Box 80.215-901 Curitiba, Paraná, Brazil
4
Cytometric Analysis. Hospital de Clínicas, Haematology Department, Federal University of Paraná, Av. General Carneiro, 181-Box 85.002-490 Curitiba, Paraná, Brazil
*
Author to whom correspondence should be addressed.
Received: 30 July 2017 / Revised: 19 September 2017 / Accepted: 28 September 2017 / Published: 19 October 2017
(This article belongs to the Special Issue Stem Cell Research)
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Abstract

Bone marrow-derived stem cells (BMDSCs) play an essential role in organ repair and regeneration. The molecular mechanisms by which hormones control BMDSCs proliferation and differentiation are unclear. Our aim in this study was to investigate how a lack of ovarian or/and thyroid hormones affects stem cell number in bone marrow lineage. To examine the effect of thyroid or/and ovarian hormones on the proliferative activity of BMDSCs, we removed the thyroid or/and the ovaries of adult female rats. An absence of ovarian and thyroid hormones was confirmed by Pap staining and Thyroid Stimulating Hormone (TSH) measurement, respectively. To obtain the stem cells from the bone marrow, we punctured the iliac crest, and aspirated and isolated cells by using a density gradient. Specific markers were used by cytometry to identify the different BMDSCs types: endothelial progenitor cells (EPCs), precursor B cells/pro-B cells, and mesenchymal stem cells (MSCs). Interestingly, our results showed that hypothyroidism caused a significant increase in the percentage of EPCs, whereas a lack of ovarian hormones significantly increased the precursor B cells/pro-B cells. Moreover, the removal of both glands led to increased MSCs. In conclusion, both ovarian and thyroid hormones appear to have key and diverse roles in regulating the proliferation of cells populations of the bone marrow. View Full-Text
Keywords: hypothyroidism; estrogen; precursor B cells/Pro-B cell; mesenchymal stem cell; endothelial progenitor cell; bone marrow; ovariectomy; thyroidectomy hypothyroidism; estrogen; precursor B cells/Pro-B cell; mesenchymal stem cell; endothelial progenitor cell; bone marrow; ovariectomy; thyroidectomy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mogharbel, B.F.; Abdelwahid, E.; Irioda, A.C.; Francisco, J.C.; Simeoni, R.B.; de Souza, D.; de Souza, C.M.C.O.; Beltrame, M.P.; Ferreira, R.J.; Guarita-Souza, L.C.; de Carvalho, K.A.T. Bone Marrow-Derived Stem Cell Populations Are Differentially Regulated by Thyroid or/and Ovarian Hormone Loss. Int. J. Mol. Sci. 2017, 18, 2139.

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