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Int. J. Mol. Sci. 2017, 18(10), 2073; doi:10.3390/ijms18102073

High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis

1
Department of Medicine, University of Udine, 33100 Udine, Italy
2
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy
3
Vita-Salute San Raffaele University, 20132 Milan, Italy
4
Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy
5
Department of Orthopedics and Traumatology, Spine Surgery III-Scoliosis Unit, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy
*
Author to whom correspondence should be addressed.
Received: 26 July 2017 / Revised: 12 September 2017 / Accepted: 25 September 2017 / Published: 29 September 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Both vitamin D and collagen have roles in osteocartilaginous homeostasis. We evaluated the association between the circulating 25-hydroxyvitamin D (25(OH)D) type I and II collagen degradation products (CTx-I, and CTx-II), and four vitamin D receptor gene (VDR) polymorphisms, in Italian males affected by low back pain (LBP) due to herniation/discopathy and/or vertebral osteochondrosis. FokI, BsmI, ApaI, and TaqI VDR-polymorphisms were detected through PCR–restriction fragment length polymorphism (RFLP), and circulating 25(OH)D, CTx-I and CTx-II were measured by immunoassays in 79 patients (of which 26 had osteochondrosis) and 79 age-, sex- and body mass index (BMI)-matched healthy controls. Among all 158 subjects, carriers of FF and Ff genotypes showed lower 25(OH)D than ff, which suggested a higher depletion of vitamin D in F allele carriers. Higher CTx-I concentrations were observed in TT versus Tt among controls, and Tt versus tt among LBP cases, which suggested a higher bone-cartilaginous catabolism in subjects bearing the T allele. Higher CTx-II concentrations were observed in patients with osteochondrosis bearing FF, bb, TT, or Aa genotypes in comparison with hernia/discopathy patients and healthy controls. Vertebral osteochondrosis shows peculiar genotypic and biochemical features related to vitamin D and the osteocartilaginous metabolism. Vitamin D has roles in the pathophysiology of osteochondrosis. View Full-Text
Keywords: adult vertebral osteochondrosis; Italian males; VDR polymorphisms; type II collagen adult vertebral osteochondrosis; Italian males; VDR polymorphisms; type II collagen
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Cauci, S.; Viganò, M.; de Girolamo, L.; De Luca, P.; Perucca Orfei, C.; Banfi, G.; Lombardi, G.; Brayda-Bruno, M.; Colombini, A. High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis. Int. J. Mol. Sci. 2017, 18, 2073.

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