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Int. J. Mol. Sci. 2017, 18(10), 2058; doi:10.3390/ijms18102058

Evaluating Changes in Cell-Wall Components Associated with Clubroot Resistance Using Fourier Transform Infrared Spectroscopy and RT-PCR

1
Canadian Light Source, 44 Innovation Blvd, Saskatoon, SK S7N 2V3, Canada
2
Currently Department of Crop Protection, Phytopathology Unit, Ecole Nationale d’Agriculture de Meknès, BP/S 40, Meknès 50001, Morocco
3
Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada 107 Science Place, Saskatoon, SK S7N 0X2, Canada
4
Currently Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India
*
Author to whom correspondence should be addressed.
Received: 18 July 2017 / Revised: 21 September 2017 / Accepted: 22 September 2017 / Published: 26 September 2017
(This article belongs to the Section Molecular Biophysics)
View Full-Text   |   Download PDF [4901 KB, uploaded 26 September 2017]   |  

Abstract

Clubroot disease is a serious threat to canola production in western Canada and many parts of the world. Rcr1 is a clubroot resistance (CR) gene identified recently and its molecular mechanisms in mediating CR have been studied using several omics approaches. The current study aimed to characterize the biochemical changes in the cell wall of canola roots connecting to key molecular mechanisms of this CR gene identified in prior studies using Fourier transform infrared (FTIR) spectroscopy. The expression of nine genes involved in phenylpropanoid metabolism was also studied using qPCR. Between susceptible (S) and resistance (R) samples, the most notable biochemical changes were related to an increased biosynthesis of lignin and phenolics. These results were supported by the transcription data on higher expression of BrPAL1. The up-regulation of PAL is indicative of an inducible defence response conferred by Rcr1; the activation of this basal defence gene via the phenylpropanoid pathway may contribute to clubroot resistance conferred by Rcr1. The data indicate that several cell-wall components, including lignin and pectin, may play a role in defence responses against clubroot. Principal components analysis of FTIR data separated non-inoculated samples from inoculated samples, but not so much between inoculated S and inoculated R samples. It is also shown that FTIR spectroscopy can be a useful tool in studying plant-pathogen interaction at cellular levels. View Full-Text
Keywords: Brassica napus; callose deposition; infrared spectroscopy; phytoalexins; quantitative RT-PCR Brassica napus; callose deposition; infrared spectroscopy; phytoalexins; quantitative RT-PCR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lahlali, R.; Song, T.; Chu, M.; Yu, F.; Kumar, S.; Karunakaran, C.; Peng, G. Evaluating Changes in Cell-Wall Components Associated with Clubroot Resistance Using Fourier Transform Infrared Spectroscopy and RT-PCR. Int. J. Mol. Sci. 2017, 18, 2058.

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