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Open AccessCommunication
Int. J. Mol. Sci. 2017, 18(10), 2035; doi:10.3390/ijms18102035

A Novel Vav3 Homolog Identified in Lamprey, Lampetra japonica, with Roles in Lipopolysaccharide-Mediated Immune Response

1,2,†
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1,2,†
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1,2
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1,2
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1,2
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1,2
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1,2,* and 1,2,*
1
College of Life Science, Liaoning Normal University, Dalian 116081, China
2
Lamprey Research Center, Liaoning Normal University, Dalian 116081, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 4 September 2017 / Revised: 19 September 2017 / Accepted: 20 September 2017 / Published: 22 September 2017
(This article belongs to the Special Issue Kinase Signal Transduction 2017)
View Full-Text   |   Download PDF [7434 KB, uploaded 26 September 2017]   |  

Abstract

Vav guanine nucleotide exchange factor 3 (Vav3), a Rho family GTPase, regulates multiple cell signaling pathways including those of T- and B-cell receptors in vertebrates through mediating the activities of the Rho family members. Whether the lamprey possesses Vav3 homolog and what role it plays in immune response remain unknown. Gene cloning, recombinant expression, antibody production and expression pattern analyses were performed to characterize the lamprey Vav3 in the current study. The lamprey Vav3 is closer to jawed vertebrates’ Vav3 molecules (about 53% identities in general) than to Vav2 molecules of jawless and jawed vertebrates (about 51% identities in general) in sequence similarity. Conserved motif analysis showed that the most distinguished parts between Vav3 and Vav2 proteins are their two Src-homology 3 domains. The relative expression levels of lamprey vav3 mRNA and protein were significantly up-regulated in lamprey lymphocytes and supraneural myeloid bodies after mixed-antigens stimulation, respectively. In addition, lamprey Vav3 were up-regulated drastically in lymphocytes and supraneural myeloid bodies after lipopolysaccharide (LPS) rather than phytohemagglutinin (PHA) stimulation. Lamprey Vav3 distributed in the cytoplasm of variable lymphocyte receptor B positive (VLRB+) lymphocytes, and the number of plasmacytes (VLRB and lamprey Vav3 double positive) in blood lymphocytes also increased after LPS stimulation. Our results proved that lamprey Vav3 was involved in the LPS-mediated immune reaction of lamprey and provided a clue for the further study of the precise role lamprey Vav3 played in the signaling pathway of lamprey VLRB+ lymphocytes. View Full-Text
Keywords: Lampetra japonica; Vav3; Phylogenetic relationship; Expression pattern; LPS-mediated immune response Lampetra japonica; Vav3; Phylogenetic relationship; Expression pattern; LPS-mediated immune response
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MDPI and ACS Style

Shen, Y.; Zhang, Y.; Han, Y.; Su, P.; Gou, M.; Pang, Y.; Li, Q.; Liu, X. A Novel Vav3 Homolog Identified in Lamprey, Lampetra japonica, with Roles in Lipopolysaccharide-Mediated Immune Response. Int. J. Mol. Sci. 2017, 18, 2035.

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