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Int. J. Mol. Sci. 2017, 18(10), 2026; doi:10.3390/ijms18102026

Cultivar and Metal-Specific Effects of Endophytic Bacteria in Helianthus tuberosus Exposed to Cd and Zn

1
Departamento de Investigación Agroambiental, IMIDRA, Finca “El Encín”, Autovía del Noreste A-2 Km 38.2, 28800 Alcalá de Henares, Madrid, Spain
2
Environmental Biology, Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, BE3590 Diepenbeek, Belgium
3
Biomedical Research Department, Hasselt University, Agoralaan building D, BE3590 Diepenbeek, Belgium
4
Department of Earth Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, UK
*
Author to whom correspondence should be addressed.
Received: 18 August 2017 / Revised: 9 September 2017 / Accepted: 12 September 2017 / Published: 21 September 2017
(This article belongs to the Special Issue Plant Microbe Interaction 2017)
View Full-Text   |   Download PDF [1996 KB, uploaded 25 September 2017]   |  

Abstract

Plant growth promoting endophytic bacteria (PGPB) isolated from Brassica napus were inoculated in two cultivars of Helianthus tuberosus (VR and D19) growing on sand supplemented with 0.1 mM Cd or 1 mM Zn. Plant growth, concentrations of metals and thiobarbituric acid (TBA) reactive compounds were determined. Colonization of roots of H. tuberosus D19 by Pseudomonas sp. 262 was evaluated using confocal laser scanning microscopy. Pseudomonas sp. 228, Serratia sp. 246 and Pseudomonas sp. 262 significantly enhanced growth of H. tuberosus D19 exposed to Cd or Zn. Pseudomonas sp. 228 significantly increased Cd concentrations in roots. Serratia sp. 246, and Pseudomonas sp. 256 and 228 resulted in significantly decreased contents of TBA reactive compounds in roots of Zn exposed D19 plants. Growth improvement and decrease of metal-induced stress were more pronounced in D19 than in VR. Pseudomonas sp. 262-green fluorescent protein (GFP) colonized the root epidermis/exodermis and also inside root hairs, indicating that an endophytic interaction was established. H. tuberosus D19 inoculated with Pseudomonas sp. 228, Serratia sp. 246 and Pseudomonas sp. 262 holds promise for sustainable biomass production in combination with phytoremediation on Cd and Zn contaminated soils. View Full-Text
Keywords: metal contaminated soil; Helianthus tuberosus; phytoremediation; high biomass crop; green fluorescent protein; plant growth promoting bacteria metal contaminated soil; Helianthus tuberosus; phytoremediation; high biomass crop; green fluorescent protein; plant growth promoting bacteria
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Montalbán, B.; Thijs, S.; Lobo, M.C.; Weyens, N.; Ameloot, M.; Vangronsveld, J.; Pérez-Sanz, A. Cultivar and Metal-Specific Effects of Endophytic Bacteria in Helianthus tuberosus Exposed to Cd and Zn. Int. J. Mol. Sci. 2017, 18, 2026.

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