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Int. J. Mol. Sci. 2017, 18(1), 5; doi:10.3390/ijms18010005

NHERF1 Enhances Cisplatin Sensitivity in Human Cervical Cancer Cells

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1,2
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4,* and 1,2,*
1
Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, China
2
Beijing International Cooperation Base for Science and Technology on China–UK Cancer Research, Beijing 100069, China
3
Experiment Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
4
Core Facilities Center, Capital Medical University, Beijing 100069, China
*
Authors to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 19 November 2016 / Revised: 9 December 2016 / Accepted: 13 December 2016 / Published: 12 January 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [3619 KB, uploaded 12 January 2017]   |  

Abstract

Cervical cancer is one of the most common female malignancies, and cisplatin-based chemotherapy is routinely utilized in locally advanced cervical cancer patients. However, resistance has been the major limitation. In this study, we found that Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) was downregulated in cisplatin-resistant cells. Analysis based on a cervical cancer dataset from The Cancer Genome Atlas (TCGA) showed association of NHERF1 expression with disease-free survival of patients received cisplatin treatment. NHERF1 overexpression inhibited proliferation and enhanced apoptosis in cisplatin-resistant HeLa cells, whereas NHERF1 knockdown had inverse effects. While parental HeLa cells were more resistant to cisplatin after NHERF1 knockdown, NHERF1 overexpression in CaSki cells promoted cisplatin sensitivity. Overexpression and knockdown studies also showed that NHERF1 significantly inhibited AKT and extracellular signal–regulated kinase (ERK) signaling pathways in cisplatin-resistant cells. Taken together, our results provide the first evidence that NHERF1 can sensitize cisplatin-refractory cervical cancer cells. This study may help to increase understanding of the molecular mechanisms underlying cisplatin resistance in tumors. View Full-Text
Keywords: cervical cancer; NHERF1; cisplatin resistance cervical cancer; NHERF1; cisplatin resistance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tao, T.; Yang, X.; Qin, Q.; Shi, W.; Wang, Q.; Yang, Y.; He, J. NHERF1 Enhances Cisplatin Sensitivity in Human Cervical Cancer Cells. Int. J. Mol. Sci. 2017, 18, 5.

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