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Int. J. Mol. Sci. 2017, 18(1), 219; doi:10.3390/ijms18010219

Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis

1
BGI-Yunnan, Kunming 650106, China
2
China National GeneBank, BGI-Shenzhen, Shenzhen 518083, China
3
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
4
College of Clinical Medicine, College of Basic Medical Sciences, Dali University, Dali 671000, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Y-h. Taguchi
Received: 14 December 2016 / Revised: 17 January 2017 / Accepted: 17 January 2017 / Published: 22 January 2017
(This article belongs to the Special Issue microRNA Regulation 2017)
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Abstract

Long dormancy period of seeds limits the large-scale artificial cultivation of the scarce Paris polyphylla var. yunnanensis, an important traditional Chinese medicine. Characterizing miRNAs and their targets is crucial to understanding the role of miRNAs during seed dormancy in this species. Considering the limited genome information of this species, we first sequenced and assembled the transcriptome data of dormant seeds and their seed coats as the reference genome. A total of 146,671 unigenes with an average length of 923 bp were identified and showed functional diversity based on different annotation methods. Two small RNA libraries from respective seeds and seed coats were sequenced and the combining data indicates that 263 conserved miRNAs belonging to at least 83 families and 768 novel miRNAs in 1174 transcripts were found. The annotations of the predicted putative targets of miRNAs suggest that these miRNAs were mainly involved in the cell, metabolism and genetic information processing by direct and indirect regulation patterns in dormant seeds of P. polyphylla var. yunnanensis. Therefore, we provide the first known miRNA profiles and their targets, which will assist with further study of the molecular mechanism of seed dormancy in P. polyphylla var. yunnanensis. View Full-Text
Keywords: Paris polyphylla var. yunnanensis; miRNAs; seed dormancy; high-throughput sequencing technology Paris polyphylla var. yunnanensis; miRNAs; seed dormancy; high-throughput sequencing technology
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Ling, L.-Z.; Zhang, S.-D.; Zhao, F.; Yang, J.-L.; Song, W.-H.; Guan, S.-M.; Li, X.-S.; Huang, Z.-J.; Cheng, L. Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis. Int. J. Mol. Sci. 2017, 18, 219.

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