Next Article in Journal
Independent Effects of a Herbivore’s Bacterial Symbionts on Its Performance and Induced Plant Defences
Next Article in Special Issue
Evidence of a DHA Signature in the Lipidome and Metabolome of Human Hepatocytes
Previous Article in Journal
The Promoting Effect of the Extracellular Matrix Peptide TNIIIA2 Derived from Tenascin-C in Colon Cancer Cell Infiltration
Previous Article in Special Issue
Recent Advances in Phospholipids from Colostrum, Milk and Dairy By-Products
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(1), 183; doi:10.3390/ijms18010183

Lipid and Glycolipid Isomer Analyses Using Ultra-High Resolution Ion Mobility Spectrometry Separations

1
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
2
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: David Arráez-Román
Received: 1 November 2016 / Revised: 19 December 2016 / Accepted: 5 January 2017 / Published: 18 January 2017
(This article belongs to the Special Issue Lipidomics and Glycomics: New Advances in Food Science and Nutrition)
View Full-Text   |   Download PDF [2192 KB, uploaded 18 January 2017]   |  

Abstract

Understanding the biological roles and mechanisms of lipids and glycolipids is challenging due to the vast number of possible isomers that may exist. Mass spectrometry (MS) measurements are currently the dominant approach for studying and providing detailed information on lipid and glycolipid presence and changes. However, difficulties in distinguishing the many structural isomers, due to the distinct lipid acyl chain positions, double bond locations or specific glycan types, inhibit the delineation and assignment of their biological roles. Here we utilized ultra-high resolution ion mobility spectrometry (IMS) separations by applying traveling waves in a serpentine multi-pass Structures for Lossless Ion Manipulations (SLIM) platform to enhance the separation of selected lipid and glycolipid isomers. The multi-pass arrangement allowed the investigation of paths ranging from ~16 m (one pass) to ~60 m (four passes) for the distinction of lipids and glycolipids with extremely small structural differences. These ultra-high resolution SLIM IMS-MS analyses provide a foundation for exploring and better understanding isomer-specific biological activities and disease processes. View Full-Text
Keywords: lipids; glycolipids; isomers; ion mobility spectrometry lipids; glycolipids; isomers; ion mobility spectrometry
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wojcik, R.; Webb, I.K.; Deng, L.; Garimella, S.V.B.; Prost, S.A.; Ibrahim, Y.M.; Baker, E.S.; Smith, R.D. Lipid and Glycolipid Isomer Analyses Using Ultra-High Resolution Ion Mobility Spectrometry Separations. Int. J. Mol. Sci. 2017, 18, 183.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top