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Int. J. Mol. Sci. 2017, 18(1), 160; doi:10.3390/ijms18010160

Roles of GATA6 during Gonadal Development in Japanese Flounder: Gonadogenesis, Regulation of Gender-Related Genes, Estrogen Formation and Gonadal Function Maintenance

Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
The authors contributed equally to this work.
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Academic Editor: Li Lin
Received: 24 November 2016 / Revised: 22 December 2016 / Accepted: 9 January 2017 / Published: 16 January 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

GATA-binding protein 6 (GATA6), a highly-conserved transcription factor of the GATA family plays an important role in gonadal cell proliferation, differentiation and endoderm development. In this study, the full-length cDNA of GATA6 of Paralichthys olivaceus (Japanese flounder) was obtained. Phylogenetic, gene structure and synteny analyses demonstrated that GATA6 of P. olivaceus is homologous to that of teleosts and tetrapods. The P. olivaceus GATA6 transcript showed higher expression in testis than in ovary, demonstrating a sexually dimorphic gene expression. During embryonic development, the expression of P. olivaceus GATA6 increased at the blastula stage, demonstrating that GATA6 is involved in morphogenesis. Results of in situ hybridization showed that GATA6 signals were detected in Sertoli cells, oogonia and oocytes. Moreover, 17α methyl testosterone, a male hormone, could moderately upregulate P. olivaceus GATA6 and downregulate P. olivaceus aromatase CYP19A1 in testis cells. These results suggest that GATA6 may play an important role in gonadal development in P. olivaceus. This study provides valuable information on the function of P. olivaceus GATA6, laying the foundation for further development of breeding techniques in this species. View Full-Text
Keywords: Paralichthys olivaceus; GATA6; dimorphic expression; hormone stimulation; gonadal development Paralichthys olivaceus; GATA6; dimorphic expression; hormone stimulation; gonadal development
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Li, Z.; Liu, X.; Sun, Y.; Liu, J.; Liu, Y.; Wang, M.; Zhang, Q.; Wang, X. Roles of GATA6 during Gonadal Development in Japanese Flounder: Gonadogenesis, Regulation of Gender-Related Genes, Estrogen Formation and Gonadal Function Maintenance. Int. J. Mol. Sci. 2017, 18, 160.

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