Potential of Pseudoshikonin I Isolated from Lithospermi Radix as Inhibitors of MMPs in IL-1β-Induced SW1353 Cells
AbstractPseudoshikonin I, the new bioactive constituent of Lithospermi radix, was isolated from this methanol extract by employing reverse-phase medium-pressure liquid chromatography (MPLC) using acetonitrile/water solvent system as eluents. The chemical structure was determined based on spectroscopic techniques, including 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHMBC, gHMQC), and QTOF/MS data. In this study, we demonstrated the effect of pseudoshikonin I on matrix-metalloproteinase (MMPs) activation and expression in interleukin (IL)-1β-induced SW1353 chondrosarcoma cells. MMPs are considered important for the maintenance of the extracellular matrix. Following treatment with PS, active MMP-1, -2, -3, -9, -13 and TIMP-2 were quantified in the SW1353 cell culture supernatants using a commercially available ELISA kit. The mRNA expression of MMPs in SW1353 cells was measured by RT-PCR. Pseudoshikonin I treatment effectively protected the activation on all tested MMPs in a dose-dependent manner. TIMP-2 mRNA expression was significantly upregulated by pseudoshikonin I treatment. Overall, we elucidated the inhibitory effect of pseudoshikonin on MMPs, and we suggest its use as a potential novel anti-osteoarthritis agent. View Full-Text
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Lee, D.Y.; Choi, S.-I.; Han, S.H.; Lee, Y.-J.; Choi, J.-G.; Lee, Y.-S.; Choi, J.H.; Lee, S.-E.; Kim, G.-S. Potential of Pseudoshikonin I Isolated from Lithospermi Radix as Inhibitors of MMPs in IL-1β-Induced SW1353 Cells. Int. J. Mol. Sci. 2016, 17, 1350.
Lee DY, Choi S-I, Han SH, Lee Y-J, Choi J-G, Lee Y-S, Choi JH, Lee S-E, Kim G-S. Potential of Pseudoshikonin I Isolated from Lithospermi Radix as Inhibitors of MMPs in IL-1β-Induced SW1353 Cells. International Journal of Molecular Sciences. 2016; 17(8):1350.Chicago/Turabian Style
Lee, Dae Y.; Choi, Soo-Im; Han, Se H.; Lee, Ye-Joo; Choi, Jong-Gil; Lee, Young-Seob; Choi, Je H.; Lee, Seung-Eun; Kim, Geum-Soog. 2016. "Potential of Pseudoshikonin I Isolated from Lithospermi Radix as Inhibitors of MMPs in IL-1β-Induced SW1353 Cells." Int. J. Mol. Sci. 17, no. 8: 1350.