Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy
AbstractDuchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disorder in which the detection of female carriers is of the utmost importance for genetic counseling. Haplotyping with polymorphic markers and quantitation of creatine kinase levels (CK) allow tracking of the at-risk haplotype and evidence muscle damage, respectively. Such approaches are useful for carrier detection in cases of unknown mutations. The lack of informative markers and the inaccuracy of CK affect carrier detection. Therefore, herein we designed novel mini-STR (Short Tandem Repeats) assays to amplify 10 loci within the DMD gene and estimated allele frequencies and the polymorphism information content among other parameters in 337 unrelated individuals from three Mexican populations. In addition, we tested the utility of the assays for carrier detection in three families. Moreover, given that serum levels of miR-206 discern between DMD patients and controls with a high area under the curve (AUC), the potential applicability for carrier detection was assessed. The serum levels of miR-206 of non-carriers (n = 24) and carriers (n = 23) were compared by relative quantitation using real-time PCR (p < 0.05), which resulted in an AUC = 0.80 in the Receiver Operating Characteristic curve analysis. In conclusion, miR-206 has potential as a “liquid biopsy” for carrier detection and genetic counseling in DMD. View Full-Text
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Anaya-Segura, M.A.; Rangel-Villalobos, H.; Martínez-Cortés, G.; Gómez-Díaz, B.; Coral-Vázquez, R.M.; Zamora-González, E.O.; García, S.; López-Hernández, L.B. Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy. Int. J. Mol. Sci. 2016, 17, 1334.
Anaya-Segura MA, Rangel-Villalobos H, Martínez-Cortés G, Gómez-Díaz B, Coral-Vázquez RM, Zamora-González EO, García S, López-Hernández LB. Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy. International Journal of Molecular Sciences. 2016; 17(8):1334.Chicago/Turabian Style
Anaya-Segura, Mónica A.; Rangel-Villalobos, Héctor; Martínez-Cortés, Gabriela; Gómez-Díaz, Benjamín; Coral-Vázquez, Ramón M.; Zamora-González, Edgar O.; García, Silvia; López-Hernández, Luz B. 2016. "Serum Levels of MicroRNA-206 and Novel Mini-STR Assays for Carrier Detection in Duchenne Muscular Dystrophy." Int. J. Mol. Sci. 17, no. 8: 1334.