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Int. J. Mol. Sci. 2016, 17(8), 1285; doi:10.3390/ijms17081285

1α,25(OH)2D3 Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition

1
Department of Toxicology, School of Public Health, Soochow University, Suzhou 215123, China
2
Department of Nutrition and Food Hygiene, Wenzhou Center for Disease Control and Prevention, Wenzhou 325000, China
3
Organ Transplant Institute, Fuzhou General Hospital, Fuzhou 350025, China
4
AIDS-STDs Prevention and Control Department, Wenzhou Center for Disease Control and Prevention, Wenzhou 325000, China
5
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, China
6
Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, China
7
Department of Radiation Biology, School of Radiation Medication and Protection, Soochow University, Suzhou 215123, China
8
Department of Labor Hygiene and Environmental Health, School of Public Health, Soochow University, Suzhou 215123, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Roman Perez-Fernandez
Received: 11 May 2016 / Revised: 12 July 2016 / Accepted: 29 July 2016 / Published: 19 August 2016
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Abstract

Ovarian cancer is the most lethal gynecological malignancy due to its high metastatic ability. Epithelial-mesenchymal transition (EMT) is essential during both follicular rupture and epithelium regeneration. However, it may also accelerate the progression of ovarian carcinomas. Experimental studies have found that 1α,25-dihydroxyvitamin-D3 [1α,25(OH)2D3] can inhibit the proliferation of ovarian cancer cells. In this study, we investigated whether 1α,25(OH)2D3 could inhibit the migration of ovarian cancer cells via regulating EMT. We established a model of transient transforming growth factor-β1(TGF-β1)-induced EMT in human ovarian adenocarcinoma cell line SKOV-3 cells. Results showed that, compared with control, 1α,25(OH)2D3 not only inhibited the migration and the invasion of SKOV-3 cells, but also promoted the acquisition of an epithelial phenotype of SKOV-3 cells treated with TGF-β1. We discovered that 1α,25(OH)2D3 increased the expression of epithelial marker E-cadherin and decreased the level of mesenchymal marker, Vimentin, which was associated with the elevated expression of VDR. Moreover, 1α,25(OH)2D3 reduced the expression level of transcription factors of EMT, such as slug, snail, and β-catenin. These results indicate that 1α,25(OH)2D3 suppresses the migration and invasion of ovarian cancer cells by inhibiting EMT, implying that 1α,25(OH)2D3 might be a potential therapeutic agent for the treatment of ovarian cancer. View Full-Text
Keywords: vitamin D; ovarian cancer; migration; EMT vitamin D; ovarian cancer; migration; EMT
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hou, Y.-F.; Gao, S.-H.; Wang, P.; Zhang, H.-M.; Liu, L.-Z.; Ye, M.-X.; Zhou, G.-M.; Zhang, Z.-L.; Li, B.-Y. 1α,25(OH)2D3 Suppresses the Migration of Ovarian Cancer SKOV-3 Cells through the Inhibition of Epithelial–Mesenchymal Transition. Int. J. Mol. Sci. 2016, 17, 1285.

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