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Int. J. Mol. Sci. 2016, 17(8), 1262; doi:10.3390/ijms17081262

Electrospun Poly(l-lactide)/Poly(ethylene glycol) Scaffolds Seeded with Human Amniotic Mesenchymal Stem Cells for Urethral Epithelium Repair

Departments of Urology and Orthopaedic Surgery, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, 708 Renmin Rd., Suzhou 215007, China
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Academic Editor: John G. Hardy
Received: 15 June 2016 / Revised: 21 July 2016 / Accepted: 27 July 2016 / Published: 9 August 2016
(This article belongs to the Section Biomaterial Sciences)
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Abstract

Tissue engineering-based urethral replacement holds potential for repairing large segmental urethral defects, which remains a great challenge at present. This study aims to explore the potential of combining biodegradable poly(l-lactide) (PLLA)/poly(ethylene glycol) (PEG) scaffolds and human amniotic mesenchymal cells (hAMSCs) for repairing urethral defects. PLLA/PEG fibrous scaffolds with various PEG fractions were fabricated via electrospinning. The scaffolds were then seeded with hAMSCs prior to implantation in New Zealand male rabbits that had 2.0 cm-long defects in the urethras. The rabbits were randomly divided into three groups. In group A, hAMSCs were grown on PLLA/PEG scaffolds for two days and then implanted to the urethral defects. In group B, only the PLLA/PEG scaffolds were used to rebuild the rabbit urethral defect. In group C, the urethral defect was reconstructed using a regular urethral reparation technique. The repair efficacy was compared among the three groups by examining the urethral morphology, tissue reconstruction, luminal patency, and complication incidence (including calculus formation, urinary fistula, and urethral stricture) using histological evaluation and urethral radiography methods. Findings from this study indicate that hAMSCs-loaded PLLA/PEG scaffolds resulted in the best urethral defect repair in rabbits, which predicts the promising application of a tissue engineering approach for urethral repair. View Full-Text
Keywords: human amniotic mesenchymal cells; electrospinning; urethral defect; tissue engineering; poly(l-lactide); poly(ethylene glycol) human amniotic mesenchymal cells; electrospinning; urethral defect; tissue engineering; poly(l-lactide); poly(ethylene glycol)
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MDPI and ACS Style

Lv, X.; Guo, Q.; Han, F.; Chen, C.; Ling, C.; Chen, W.; Li, B. Electrospun Poly(l-lactide)/Poly(ethylene glycol) Scaffolds Seeded with Human Amniotic Mesenchymal Stem Cells for Urethral Epithelium Repair. Int. J. Mol. Sci. 2016, 17, 1262.

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