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Int. J. Mol. Sci. 2016, 17(8), 1237; doi:10.3390/ijms17081237

VEGF and FGF2 Improve Revascularization, Survival, and Oocyte Quality of Cryopreserved, Subcutaneously-Transplanted Mouse Ovarian Tissues

1
Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan
2
Mackay Junior College of Medicine, Nursing, and Management, Beitou District, Taipei City 112, Taiwan
3
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan
4
Mackay Medical College, Sanzhi District, New Taipei City 252, Taiwan
5
Department of Obstetrics and Gynecology, Taipei Medical University, Taipei City 110, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Muraca
Received: 8 May 2016 / Revised: 25 July 2016 / Accepted: 26 July 2016 / Published: 30 July 2016
(This article belongs to the Special Issue Advances in Cell Transplantation)
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Abstract

This study was conducted to investigate the effect of the vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF2) on revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue. Autologous subcutaneous transplantation of vitrified-thawed mouse ovarian tissues treated with (experimental group) or without (control group) VEGF and FGF2 was performed. After transplantation to the inguinal region for two or three weeks, graft survival, angiogenesis, follicle development, and oocyte quality were examined after gonadotropin administration. VEGF coupled with FGF2 (VEGF/FGF2) promoted revascularization and significantly increased the survival rate of subcutaneously-transplanted cryopreserved ovarian tissues compared with untreated controls. The two growth factors did not show long-term effects on the ovarian grafts. In contrast to the untreated ovarian grafts, active folliculogenesis was revealed as the number of follicles at various stages and of mature oocytes in antral follicles after gonadotropin administration were remarkably higher in the VEGF/FGF2-treated groups. Although the fertilization rate was similar between the VEGF/FGF2 and control groups, the oocyte quality was much better in the VEGF/FGF2-treated grafts as demonstrated by the higher ratio of blastocyst development. Introducing angiogenic factors, such as VEGF and FGF2, may be a promising strategy to improve revascularization, survival, and oocyte quality of cryopreserved, subcutaneously-transplanted mouse ovarian tissue. View Full-Text
Keywords: angiogenic factor; ovarian cryopreservation; ovarian transplantation; oocyte quality; fertility reservation angiogenic factor; ovarian cryopreservation; ovarian transplantation; oocyte quality; fertility reservation
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MDPI and ACS Style

Li, S.-H.; Hwu, Y.-M.; Lu, C.-H.; Chang, H.-H.; Hsieh, C.-E.; Lee, R.K.-K. VEGF and FGF2 Improve Revascularization, Survival, and Oocyte Quality of Cryopreserved, Subcutaneously-Transplanted Mouse Ovarian Tissues. Int. J. Mol. Sci. 2016, 17, 1237.

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