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Int. J. Mol. Sci. 2016, 17(7), 959; doi:10.3390/ijms17070959

MicroRNA-21a-5p Functions on the Regulation of Melanogenesis by Targeting Sox5 in Mouse Skin Melanocytes

1
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, Shanxi, China
2
Wujiang River Institute of Agriculture & Forestry Economics, Tongren University, Tongren 554300, Guizhou, China
*
Author to whom correspondence should be addressed.
Academic Editor: Y-h. Taguchi
Received: 29 February 2016 / Revised: 2 June 2016 / Accepted: 12 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue MicroRNA Regulation)
View Full-Text   |   Download PDF [1245 KB, uploaded 24 June 2016]   |  

Abstract

MicroRNAs (miRNAs) play an important role in regulating almost all biological processes. miRNAs bind to the 3′ untranslated region (UTR) of mRNAs by sequence matching. In a previous study, we demonstrated that miR-21 was differently expressed in alpaca skin with different hair color. However, the molecular and cellular mechanisms for miR-21 to regulate the coat color are not yet completely understood. In this study, we transfected miR-21a-5p into mouse melanocytes and demonstrated its function on melanogenesis of miR-21a-5p by targeting Sox5, which inhibits melanogenesis in mouse melanocytes. The results suggested that miR-21a-5p targeted Sox5 gene based on the binding site in 3′ UTR of Sox5 and overexpression of miR-21a-5p significantly down-regulated Sox5 mRNA and protein expression. Meanwhile, mRNA and protein expression of microphthalmia transcription factor (MITF) and Tyrosinase (TYR) were up-regulated, which subsequently make the melanin production in melanocytes increased. The results suggest that miR-21a-5p regulates melanogenesis via MITF by targeting Sox5. View Full-Text
Keywords: melanocytes; Sox5; miR-21a-5p; transcriptional regulation melanocytes; Sox5; miR-21a-5p; transcriptional regulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, P.; Zhao, Y.; Fan, R.; Chen, T.; Dong, C. MicroRNA-21a-5p Functions on the Regulation of Melanogenesis by Targeting Sox5 in Mouse Skin Melanocytes. Int. J. Mol. Sci. 2016, 17, 959.

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