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Int. J. Mol. Sci. 2016, 17(7), 1115; doi:10.3390/ijms17071115

Structural Characterization and Evolutionary Relationship of High-Molecular-Weight Glutenin Subunit Genes in Roegneria nakaii and Roegneria alashanica

1
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 8 May 2016 / Revised: 28 June 2016 / Accepted: 4 July 2016 / Published: 19 July 2016
(This article belongs to the Section Molecular Botany)
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Abstract

The Roegneria of Triticeae is a large genus including about 130 allopolyploid species. Little is known about its high-molecular-weight glutenin subunits (HMW-GSs). Here, we reported six novel HMW-GS genes from R. nakaii and R. alashanica. Sequencing indicated that Rny1, Rny3, and Ray1 possessed intact open reading frames (ORFs), whereas Rny2, Rny4, and Ray2 harbored in-frame stop codons. All of the six genes possessed a similar primary structure to known HMW-GS, while showing some unique characteristics. Their coding regions were significantly shorter than Glu-1 genes in wheat. The amino acid sequences revealed that all of the six genes were intermediate towards the y-type. The phylogenetic analysis showed that the HMW-GSs from species with St, StY, or StH genome(s) clustered in an independent clade, varying from the typical x- and y-type clusters. Thus, the Glu-1 locus in R. nakaii and R. alashanica is a very primitive glutenin locus across evolution. The six genes were phylogenetically split into two groups clustered to different clades, respectively, each of the two clades included the HMW-GSs from species with St (diploid and tetraploid species), StY, and StH genomes. Hence, it is concluded that the six Roegneria HMW-GS genes are from two St genomes undergoing slight differentiation. View Full-Text
Keywords: Roegneria nakaii; Roegneria alashanica; high-molecular-weight glutenin subunits; gene cloning; variation; phylogenetic analysis Roegneria nakaii; Roegneria alashanica; high-molecular-weight glutenin subunits; gene cloning; variation; phylogenetic analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhang, L.; Li, Z.; Fan, R.; Wei, B.; Zhang, X. Structural Characterization and Evolutionary Relationship of High-Molecular-Weight Glutenin Subunit Genes in Roegneria nakaii and Roegneria alashanica. Int. J. Mol. Sci. 2016, 17, 1115.

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