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Int. J. Mol. Sci. 2016, 17(6), 992; doi:10.3390/ijms17060992

Molecular Characteristics of Methicillin-Resistant Staphylococcus epidermidis on the Abdominal Skin of Females before Laparotomy

1
Medical Genetics, West China Shool of Medicine, West China Hospital, Sichuan University, Chengdu 610041, China
2
School of Medical Laboratory Science, Chengdu Medical College, Chengdu 610500, China
3
Department of Laboratory Medicine, Sichuan Provincial Hospital for Women and Children, Chengdu 610045, China
*
Author to whom correspondence should be addressed.
Academic Editor: Chris Jackson
Received: 1 April 2016 / Revised: 6 June 2016 / Accepted: 20 June 2016 / Published: 22 June 2016
(This article belongs to the Special Issue Inflammatory Skin Conditions)
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Abstract

Staphylococcus epidermidis, especially methicillin-resistant strains, may be the source of surgical site infections and may be a reservoir of staphylococcal cassette chromosome mec (SCCmec) for S. aureus. The aim of this study was to investigate the prevalence of methicillin-resistant S. epidermidis (MRSE) on the abdominal skin of females before laparotomy and determine the molecular characteristics and antimicrobial susceptibility patterns of these isolates. MRSE was found in 54 of 157 isolates based on mecA gene detection, and there was no difference in icaA gene carriage rate between MRSE and methicillin-susceptible S. epidermidis (MSSE) isolates. Antimicrobial susceptibility profiles were determined by broth microdilution antimicrobial susceptibility testing according to the latest CLSI manuals. All MRSE isolates had unfavorable antimicrobial susceptibility patterns. Twenty-three MRSE strains (42.6%) were multi-drug resistant. SCCmec typing and pulsed field gel electrophoresis (PFGE) typing was performed. Thirty-nine (72.2%) had a single SCCmec type, whereas 1.9% had two types. Fourteen strains (25.9%) were non-typeable (NT). The most frequent MRSE genotype was SCCmec type IVa. High diversity with PFGE patterns was obtained for MRSE, and there were no isolates exhibiting identical pulsotype. The results confirm that methicillin-resistant strains are frequently present among S. epidermidis on the abdominal skin of females before laparotomy. Moreover, resistance profiles seem to have no association with the SCCmec types or PFGE types for most common antibiotics. View Full-Text
Keywords: methicillin-resistant Staphylococcus epidermidis; SCCmec; PFGE; skin; laparotomy; antibiotic resistant methicillin-resistant Staphylococcus epidermidis; SCCmec; PFGE; skin; laparotomy; antibiotic resistant
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Wang, P.-J.; Xie, C.-B.; Sun, F.-H.; Guo, L.-J.; Dai, M.; Cheng, X.; Ma, Y.-X. Molecular Characteristics of Methicillin-Resistant Staphylococcus epidermidis on the Abdominal Skin of Females before Laparotomy. Int. J. Mol. Sci. 2016, 17, 992.

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