Next Article in Journal
Tetrapyrroles as Endogenous TSPO Ligands in Eukaryotes and Prokaryotes: Comparisons with Synthetic Ligands
Previous Article in Journal
Molecular Characterization of the Complete Genome of Three Basal-BR Isolates of Turnip mosaic virus Infecting Raphanus sativus in China
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2016, 17(6), 884; doi:10.3390/ijms17060884

Development of EST Intron-Targeting SNP Markers for Panax ginseng and Their Application to Cultivar Authentication

1
School of Life Sciences, Yantai University, Yantai 264005, China
2
School of Pharmacy, Yantai University, Yantai 264005, China
3
Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Gyunggi-do 446-701, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 16 March 2016 / Revised: 19 May 2016 / Accepted: 26 May 2016 / Published: 4 June 2016
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [3631 KB, uploaded 4 June 2016]   |  

Abstract

Panax ginseng is one of the most valuable medicinal plants in the Orient. The low level of genetic variation has limited the application of molecular markers for cultivar authentication and marker-assisted selection in cultivated ginseng. To exploit DNA polymorphism within ginseng cultivars, ginseng expressed sequence tags (ESTs) were searched against the potential intron polymorphism (PIP) database to predict the positions of introns. Intron-flanking primers were then designed in conserved exon regions and used to amplify across the more variable introns. Sequencing results showed that single nucleotide polymorphisms (SNPs), as well as indels, were detected in four EST-derived introns, and SNP markers specific to “Gopoong” and “K-1” were first reported in this study. Based on cultivar-specific SNP sites, allele-specific polymerase chain reaction (PCR) was conducted and proved to be effective for the authentication of ginseng cultivars. Additionally, the combination of a simple NaOH-Tris DNA isolation method and real-time allele-specific PCR assay enabled the high throughput selection of cultivars from ginseng fields. The established real-time allele-specific PCR assay should be applied to molecular authentication and marker assisted selection of P. ginseng cultivars, and the EST intron-targeting strategy will provide a potential approach for marker development in species without whole genomic DNA sequence information. View Full-Text
Keywords: Panax ginseng; intron-targeting; cultivar authentication; SNP; real-time allele-specific PCR Panax ginseng; intron-targeting; cultivar authentication; SNP; real-time allele-specific PCR
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, H.; Li, G.; Kwon, W.-S.; Yang, D.-C. Development of EST Intron-Targeting SNP Markers for Panax ginseng and Their Application to Cultivar Authentication. Int. J. Mol. Sci. 2016, 17, 884.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top