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Int. J. Mol. Sci. 2016, 17(5), 787; doi:10.3390/ijms17050787

Functional and Biochemical Characterization of Three Recombinant Human Glucose-6-Phosphate Dehydrogenase Mutants: Zacatecas, Vanua-Lava and Viangchan

1
Laboratorio de Bioquímica Genética, Instituto Nacional de Pediatría, México D.F. 04530, Mexico
2
CONACYT—Instituto Nacional de Pediatría, México D.F. 04530, Mexico
3
Laboratorio de Neurociencias, Instituto Nacional de Pediatría, México D.F. 04530, Mexico
4
CONACYT—Laboratorio de Bioingeniería, Universidad de Colima, Colima 28400, Mexico
5
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico
6
Laboratorio de Inmunoquímica, Hospital Infantil de México Federico Gómez, México D.F. 06720, Mexico
7
Colegio de Ciencias y Humanidades, Plantel Casa Libertad, UACM, México D.F. 09620, Mexico
8
Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, México D.F. 04510, Mexico
*
Authors to whom correspondence should be addressed.
Academic Editor: Christo Z. Christov
Received: 21 April 2016 / Revised: 21 April 2016 / Accepted: 16 May 2016 / Published: 21 May 2016
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Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency in humans causes severe disease, varying from mostly asymptomatic individuals to patients showing neonatal jaundice, acute hemolysis episodes or chronic nonspherocytic hemolytic anemia. In order to understand the effect of the mutations in G6PD gene function and its relation with G6PD deficiency severity, we report the construction, cloning and expression as well as the detailed kinetic and stability characterization of three purified clinical variants of G6PD that present in the Mexican population: G6PD Zacatecas (Class I), Vanua-Lava (Class II) and Viangchan (Class II). For all the G6PD mutants, we obtained low purification yield and altered kinetic parameters compared with Wild Type (WT). Our results show that the mutations, regardless of the distance from the active site where they are located, affect the catalytic properties and structural parameters and that these changes could be associated with the clinical presentation of the deficiency. Specifically, the structural characterization of the G6PD Zacatecas mutant suggests that the R257L mutation have a strong effect on the global stability of G6PD favoring an unstable active site. Using computational analysis, we offer a molecular explanation of the effects of these mutations on the active site. View Full-Text
Keywords: glucose-6-phosphate dehydrogenase (G6PD) deficiency; human G6PD mutants; steady state kinetics; thermostability; structural characterization glucose-6-phosphate dehydrogenase (G6PD) deficiency; human G6PD mutants; steady state kinetics; thermostability; structural characterization
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Gómez-Manzo, S.; Marcial-Quino, J.; Vanoye-Carlo, A.; Serrano-Posada, H.; González-Valdez, A.; Martínez-Rosas, V.; Hernández-Ochoa, B.; Sierra-Palacios, E.; Castillo-Rodríguez, R.A.; Cuevas-Cruz, M.; Rodríguez-Bustamante, E.; Arreguin-Espinosa, R. Functional and Biochemical Characterization of Three Recombinant Human Glucose-6-Phosphate Dehydrogenase Mutants: Zacatecas, Vanua-Lava and Viangchan. Int. J. Mol. Sci. 2016, 17, 787.

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