A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization
AbstractLaccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1) was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight days, 26 °C, 18% (w/w) milled corncob, 0.8% (w/w) NH4Cl and 50 mmol·L−1 CuSO4, initial moisture 4.1 mL·g−1), the laccase activity reached 138.6 ± 13.2 U·g−1. Lacps1 was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum pH and temperature for the oxidation of 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4 and 74.4 °C, respectively. Lacps1 was stable at pH 3.0–8.0, and after two hours at 55–60 °C, presenting high redox potential (0.747 V vs. NHE). ABTS was oxidized with an apparent affinity constant of 147.0 ± 6.4 μmol·L−1, maximum velocity of 413.4 ± 21.2 U·mg−1 and catalytic efficiency of 3140.1 ± 149.6 L·mmol−1·s−1. The maximum decolorization percentages of bromophenol blue (BPB), remazol brilliant blue R and reactive blue 4 (RB4), at 25 or 40 °C without redox mediators, reached 90%, 80% and 60%, respectively, using either pure Lacps1 or the crude extract. This is the first study of the decolorization of BPB and RB4 by a P. sanguineus laccase. The data suggested good potential for treatment of industrial dye-containing effluents. View Full-Text
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Zimbardi, A.L.R.L.; Camargo, P.F.; Carli, S.; Aquino Neto, S.; Meleiro, L.P.; Rosa, J.C.; De Andrade, A.R.; Jorge, J.A.; Furriel, R.P.M. A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization. Int. J. Mol. Sci. 2016, 17, 672.
Zimbardi ALRL, Camargo PF, Carli S, Aquino Neto S, Meleiro LP, Rosa JC, De Andrade AR, Jorge JA, Furriel RPM. A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization. International Journal of Molecular Sciences. 2016; 17(5):672.Chicago/Turabian Style
Zimbardi, Ana L.R.L.; Camargo, Priscila F.; Carli, Sibeli; Aquino Neto, Sidney; Meleiro, Luana P.; Rosa, Jose C.; De Andrade, Adalgisa R.; Jorge, João A.; Furriel, Rosa P.M. 2016. "A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization." Int. J. Mol. Sci. 17, no. 5: 672.
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