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Int. J. Mol. Sci. 2016, 17(4), 581; doi:10.3390/ijms17040581

Proteomic and Microscopic Strategies towards the Analysis of the Cytoskeletal Networks in Major Neuropsychiatric Disorders

1
School of Rural Medicine, University of New England, Armidale, NSW 2351, Australia
2
Center for Bioactive Discovery in Health and Aging, School of Science and Technology, University of New England, Armidale, NSW 2351, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Kenji Hashimoto
Received: 11 March 2016 / Revised: 6 April 2016 / Accepted: 8 April 2016 / Published: 20 April 2016
(This article belongs to the Collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [519 KB, uploaded 20 April 2016]   |  

Abstract

Mental health disorders have become worldwide health priorities. It is estimated that in the next 20 years they will account for a 16 trillion United State dollars (US$) loss. Up to now, the underlying pathophysiology of psychiatric disorders remains elusive. Altered cytoskeleton proteins expression that may influence the assembly, organization and maintenance of cytoskeletal integrity has been reported in major depressive disorders, schizophrenia and to some extent bipolar disorders. The use of quantitative proteomics, dynamic microscopy and super-resolution microscopy to investigate disease-specific protein signatures holds great promise to improve our understanding of these disorders. In this review, we present the currently available quantitative proteomic approaches use in neurology, gel-based, stable isotope-labelling and label-free methodologies and evaluate their strengths and limitations. We also reported on enrichment/subfractionation methods that target the cytoskeleton associated proteins and discuss the need of alternative methods for further characterization of the neurocytoskeletal proteome. Finally, we present live cell imaging approaches and emerging dynamic microscopy technology that will provide the tools necessary to investigate protein interactions and their dynamics in the whole cells. While these areas of research are still in their infancy, they offer huge potential towards the understanding of the neuronal network stability and its modification across neuropsychiatric disorders. View Full-Text
Keywords: neuropsychiatric disorders; cytoskeleton; cytoskeleton associated proteins; proteomics methodologies; cytoskeleton protein enrichment; live cell imaging; super-resolution microscopy neuropsychiatric disorders; cytoskeleton; cytoskeleton associated proteins; proteomics methodologies; cytoskeleton protein enrichment; live cell imaging; super-resolution microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Coumans, J.V.F.; Palanisamy, S.K.A.; McFarlane, J.; Moens, P.D.J. Proteomic and Microscopic Strategies towards the Analysis of the Cytoskeletal Networks in Major Neuropsychiatric Disorders. Int. J. Mol. Sci. 2016, 17, 581.

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